傣药“雅解”：宾蒿、傣百解及竹叶兰的生物活性及其解毒机制

张雪飞; 赵俊可; 郝素旗; 丁青; 于世慧; 伊木朗; 王军; 胡海燕

doi:10.13471/j.cnki.acta.snus.2022E027

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傣药“雅解”：宾蒿、傣百解及竹叶兰的生物活性及其解毒机制

研究论文 | 更新时间：2023-11-01

- 傣药“雅解”：宾蒿、傣百解及竹叶兰的生物活性及其解毒机制
- Biological activities and detoxification mechanisms of Clerodendrum chinense var. simplex, Marsdenia tenacissima and Arundina graminifolia: The Dai antidotes
- 中山大学学报(自然科学版)(中英文) 2023年62卷第3期页码：89-99
- 作者机构：
  
  1.滇西应用技术大学傣医药学院，云南景洪 666100
  2.中山大学药学院，广东广州 510006
  3.云南傣药有限公司，云南瑞丽 678699
- 作者简介：
  
  ZHANG Xuefei E-mail：（zhangxf27@mail2.sysu.edu.cn）
  ZHAO Junke E-mail：（1623928099@qq.com）
  HAO Suqi E-mail：（1475338624@qq.com）
  DING Qing E-mail：（dq4115222@163.com）
  YU Shihui E-mail：（956800457@qq.com）
  MOHAMMAD Imran shair E-mail：（shair@mail.sysu.edu.cn）
  WANG Jun E-mail：（wjun@mail.sysu.edu.cn）
  [ "HU Haiyan（lsshhy@mail.sysu.edu.cn）" ]
- 基金信息：
  
  Yunnan Provincial Department of Education Science Research Fund Project(2018JS709);National Natural Science Foundation of China(81973264);Guangdong Basic and Applied Basic Research Foundation(2019A1515011954;2020A1515010593)
- DOI：10.13471/j.cnki.acta.snus.2022E027
  中图分类号： R29
- 纸质出版日期：2023-05-25，
  
  网络出版日期：2023-01-30，
  
  收稿日期：2022-09-13，
  
  录用日期：2022-11-04
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张雪飞,赵俊可,郝素旗等.傣药“雅解”：宾蒿、傣百解及竹叶兰的生物活性及其解毒机制[J].中山大学学报(自然科学版),2023,62(03):89-99.

ZHANG Xuefei,ZHAO Junke,HAO Suqi,et al.Biological activities and detoxification mechanisms of Clerodendrum chinense var. simplex, Marsdenia tenacissima and Arundina graminifolia: The Dai antidotes[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(03):89-99.
张雪飞,赵俊可,郝素旗等.傣药“雅解”：宾蒿、傣百解及竹叶兰的生物活性及其解毒机制[J].中山大学学报(自然科学版),2023,62(03):89-99. DOI： 10.13471/j.cnki.acta.snus.2022E027.

ZHANG Xuefei,ZHAO Junke,HAO Suqi,et al.Biological activities and detoxification mechanisms of Clerodendrum chinense var. simplex, Marsdenia tenacissima and Arundina graminifolia: The Dai antidotes[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(03):89-99. DOI： 10.13471/j.cnki.acta.snus.2022E027.

摘要

傣药“雅解”是傣族传统医学中最具特色的药物和治疗方法。其中，宾蒿（

Clerodendrum chinense

var.

simplex

）、傣百解（

Marsdenia tenacissima

）和竹叶兰（

Arundina graminifolia

）作为3种常见的“雅解”被广泛使用。本研究使用石油醚、乙酸乙酯、正丁醇或水依次萃取3种“雅解”的乙醇提取物获得各极性部位，并制备3种“雅解”的水煎剂。经测定3种“雅解”的总黄酮质量分数为22.41～586.39 mg/g，高于总多酚质量分数（2.76～28.66 mg/g）。3种“雅解”的提取物均可清除 DPPH、·OH 和 ABTS自由基，其中对ABTS自由基的清除率高于其他自由基（IC

380 μg/mL）。它们对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抑制作用较弱。此外，3种“雅解”具有一定的抗炎作用，在60 μg/mL 的质量浓度下即可显著下调脂多糖刺激巨噬细胞产生的NO、肿瘤坏死因子-α、白细胞介素-1β 和白细胞介素-6。综上所述，本研究通过现代科学手段初步揭示了3种“雅解”的解毒作用机理，为“雅解”的进一步优化与研究奠定了基础。

Abstract

Dai antidotes are the most distinctive medicine and treatment in traditional Dai medicine.

Bin Hao

(

Clerodendrum chinense

var.

simplex

)

Dai Bai Jie

(

Marsdenia tenacissima

)

and

Zhu Ye Lan

(

Arundina graminifolia

) are three Dai antidotes widely used for their "detoxifying effects"

and their use is rooted in a theoretical system significantly different and much less understood than Western or traditional Chinese medicines. Here

we successively extracted the three Dai antidotes using petroleum ether

ethyl acetate

-butanol

or water

and then prepared their decoctions. The content of total flavonoids in three Dai antidotes ranged from 22.41 to 586.39 mg/g

which is higher than the content of total polyphenols (2.76 to 28.66 mg/g). The various extracts were found to scavenge radicals of DPPH

·OH and ABTS. They scavenged ABTS radicals much more efficiently than other radicals (IC

380 μg/mL). They weakly inhibited the growth of

E. coli

P. aeruginosa

and

S. aureus.

Notably

even at low concentration 60 μg/mL

the extracts can significantly down-regulate the production of NO

TNF-α

IL-1β

and IL-6 by macrophages stimulated with LPS. In conclusion

our results provide the first mechanistic insights into the detoxifying effects of three Dai antidotes

providing a foundation for their optimization and for future research to strengthen Dai medicine through modern scientific practices.

关键词

傣药“雅解”宾蒿（Clerodendrum chinense var. simplex）傣百解（Marsdenia tenacissima）竹叶兰（Arundina graminifolia）解毒作用

Keywords

Dai antidoteClerodendrum chinense var. simplexMarsdenia tenacissimaArundina graminifoliadetoxification

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