有害生物控制与资源利用国家重点实验室 / 中山大学生命科学学院,广东 广州 510275
杨梅(1997年生),女;研究方向:昆虫生物化学与分子生物学;E-mail:fengb27@mail2.sysu.edu.cn
王方海(1965年生),男;研究方向:昆虫生物化学与分子生物学;E-mail:lsswfh@mail.sysu.edu.cn
纸质出版日期:2023-03-25,
网络出版日期:2022-11-08,
收稿日期:2022-07-08,
录用日期:2022-08-05
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杨梅,冯博,王方海.白背飞虱ttk结构特点及在若虫中的表达[J].中山大学学报(自然科学版),2023,62(02):47-50.
YANG Mei,FENG Bo,WANG Fanghai.The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(02):47-50.
杨梅,冯博,王方海.白背飞虱ttk结构特点及在若虫中的表达[J].中山大学学报(自然科学版),2023,62(02):47-50. DOI: 10.13471/j.cnki.acta.snus.2022E019.
YANG Mei,FENG Bo,WANG Fanghai.The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(02):47-50. DOI: 10.13471/j.cnki.acta.snus.2022E019.
ttk
基因转录产物为重要的转录因子,可以调控生物体的发育、分化、性行为等。本研究从自建的转录组数据库中比对到一条来源于白背飞虱(
Sogatella furcifera
)
ttk
的cDNA序列,全长为1 657 bp,其开放阅读框序列为1 377 bp,编码由458个氨基酸组成的蛋白质,具有典型的BTB结构域和锌指结构。系统进化树显示白背飞虱
ttk
与六星灯蛾(
Zygaena filipendulae
)、温带臭虫(
Cimex lectularius
)和褐飞虱(
Nilaparvata lugens
)的同源性较近。定量检测结果显示,
ttk
在1龄若虫期含量较高,2龄则下降到较低的水平,从3龄开始,随着龄期的增长,表达量则逐渐增加,到5龄若虫时,
ttk
表达量是3龄若虫的2倍多。这是第一次详细报道白背飞虱
ttk
基因的结构和表达水平,为今后进一步研究该基因的具体生物学功能提供了基础。
Tramtrack(
ttk
) gene transcription products are important transcription factors, which can regulate the development, differentiation and sexual behavior of organisms. In this study, a 1 657 bp cDNA sequence of
ttk
was identified from the transcriptome database of
Sogatella furcifera
. The open reading frame is 1 377 bp in length and encodes a protein composed of 458 amino acids. It has a typical BTB domain and a zinc finger structure. The phylogenetic tree shows that
ttk
of
S. furcifera
is closely homologous to that of
Zygaena filipendulae
,
Cimex lectularius
and
Nilaparvata lugens
. Quantitative detection results showed that the transcriptional level of
ttk
was higher in the 1st instar nymphs, but decreased to a lower level in the 2nd instar nymphs. From the 3rd instar,
ttk
expression increased gradually with instar growth, and at the 5th instar nymphs,
ttk
expression was more than twice as high as that in the 3rd instar nymphs. This is the first in detail investigation on
ttk
gene structure of white backed planthopper, and the results provide a foundation for further and function and regulation study of
ttk
gene, in the growth and development of rice planthopper in the future.
tramtrack(ttk)白背飞虱(Sogatella furcifera)若虫基因表达
tramtrack(ttk)Sogatella furciferanymphgene expression
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