1.广西中医药大学海洋药物研究院 / 药学院,广西 南宁 530200
2.广西壮瑶药工程技术研究中心,广西 南宁 530200
3.广西师范大学化学与药学学院, 广西 桂林 541000
肖泽恩(1987年生),女;研究方向:天然药物化学;E-mail:xiaozeen@mail2.sysu.edu.cn
谭振(1986年生),男;研究方向:天然药物化学;E-mail:tanzhen@gxtcmu.edu.cn
刘永宏(1972年生),男;研究方向:海洋创新药物;E-mail:yonghongliu@scsio.ac.cn
纸质出版日期:2022-11-25,
网络出版日期:2022-03-30,
收稿日期:2022-02-18,
录用日期:2022-03-08
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肖泽恩,黄锡山,韦蔚烜等.北海红树林内生真菌Aspergillus sp. GXIMD00016的抗炎次级代谢产物[J].中山大学学报(自然科学版),2022,61(06):69-73.
XIAO Ze′en,HUANG Xishan,WEI Weixuan,et al.Anti-inflammatory secondary metabolites from a Beihai mangrove endophytic fungus Aspergillus sp. GXIMD00016[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(06):69-73.
肖泽恩,黄锡山,韦蔚烜等.北海红树林内生真菌Aspergillus sp. GXIMD00016的抗炎次级代谢产物[J].中山大学学报(自然科学版),2022,61(06):69-73. DOI: 10.13471/j.cnki.acta.snus.2022E006.
XIAO Ze′en,HUANG Xishan,WEI Weixuan,et al.Anti-inflammatory secondary metabolites from a Beihai mangrove endophytic fungus Aspergillus sp. GXIMD00016[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(06):69-73. DOI: 10.13471/j.cnki.acta.snus.2022E006.
采用大米培养基对红树内生真菌
Aspergillus
sp. GXIMD00016进行规模发酵,利用层析技术对发酵产物进行分离纯化,获得7个化合物。经波谱技术鉴定结构,并通过与文献对比鉴定化合物为aculeatusquinone A(
1
)、aculeatusquinone C(
2
)、roseopurpurin A(
3
)、myxotrichin C(
4
)、2,7-didechlorovicanic(
5
)、atraric acid(
6
)和2,5-dimethyl-1,3-benzenediol(
7
),其中化合物
5
为新天然产物,化合物
3
和
4
为首次从曲霉菌属中获得。首次考察化合物
1
~
3
和
5
~
7
对脂多糖 (lipopolysaccharide,LPS) 诱导RAW 264.7细胞产生NO的抑制作用,化合物
1
~
3
具有显著抗炎作用,IC
50
值为16.68~28.15 μmol/L。
The mangrove endophytic fungus
Aspergillus
sp. GXIMD00016 was fermented by using rice medium. The metabolites were isolated by the chromatography technology and seven compounds were obtained. By analyzing MS and NMR spectroscopic data,their structures were identified as aculeatusquinone A
(1
), aculeatusquinone C (
2
), roseopurpurin A (
3
), myxotrichin C (
4
), 2,7-didechlorovicanic (
5
), atraric acid (
6
) and 2,5-dimethyl-1,3-benzenediol (
7
). Besides, compound
5
was a new natural product, and compounds
3
and
4
were isolated from
Aspergillus
sp. for the first time. The inhibitory effects of compounds
1
-
3
and
5
-
7
on lipopolysaccharide (LPS) induced NO production were evaluated in mouse macrophage RAW264.7 cells. The results showed that compounds
1
-
3
had a significant anti-inflammatory activity with IC
50
values ranging from 16.68 to 28.15 μmol/L.
红树内生真菌次级代谢产物曲霉抗炎苯醌
mangrove endophytic fungussecondary metabolitesAspergillusanti-inflammatorybenzoquinone
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