1.广东海洋大学食品科技学院,广东 湛江 524088
2.中山大学化学学院,广东 广州 510006
刘亚月(1989年生),女;研究方向:海洋生物活性物质;E-mail:yayue_liu@163.com
佘志刚(1963年生),男;研究方向:天然产物化学;E-mail:cesshzhg@mail.sysu.edu.cn
纸质出版日期:2022-09-25,
网络出版日期:2022-01-21,
收稿日期:2021-11-04,
录用日期:2021-12-15
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刘亚月,吴颖楠,佘志刚.无瓣海桑来源内生真菌Aspergillus sp.HNY16-5B中的二聚呫吨类化合物[J].中山大学学报(自然科学版),2022,61(05):76-83.
LIU Yayue,WU Yinnan,SHE Zhigang.The dimeric xanthones of mangrove endophytic fungus Aspergillus sp. HNY16-5B isolated from Sonneratia apetala[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(05):76-83.
刘亚月,吴颖楠,佘志刚.无瓣海桑来源内生真菌Aspergillus sp.HNY16-5B中的二聚呫吨类化合物[J].中山大学学报(自然科学版),2022,61(05):76-83. DOI: 10.13471/j.cnki.acta.snus.2021C025.
LIU Yayue,WU Yinnan,SHE Zhigang.The dimeric xanthones of mangrove endophytic fungus Aspergillus sp. HNY16-5B isolated from Sonneratia apetala[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(05):76-83. DOI: 10.13471/j.cnki.acta.snus.2021C025.
研究了海南红树植物无瓣海桑
Sonneratia apetala
来源内生真菌
Aspergillus
sp.(HNY16-5B)的次级代谢产物。利用察氏培养基对该菌株进行发酵培养,并综合运用正、反相硅胶柱层析,Sephadex LH-20凝胶和重结晶等分离手段,从中分离获得了5个二聚呫吨化合物(
1
~
5
)。运用MS和1D、2D NMR等现代波谱技术,并与文献数据对比,鉴定为:asperpyrone B (
1
)、fonsecinone A (
2
)、aurasperone A (
3
)、asperpyrone A (
4
)和dianhydroaurasperone C (
5
)。通过培养成功获得了化合物
2
的单晶,并利用铜靶X-Ray单晶衍射技术确定其轴手性为
S
。通过对比CD谱,最终确定了化合物
1
~
5
的轴手性均为
S
。
The secondary metabolites of endophytic fungus
Aspergillu
s sp. (HNY16-5B), isolated from the leaves of the mangrove plant
Sonneratia apetala
, were studied. The strain was fermented and cultured with czapek′s medium, and then subjected to silica gel column chromatography, Sephadex LH-20, ODS, and recrystallization. Five dimerxanthones: asperpyrones B (
1
), fonsecinone A (
2
), aurasperone A (
3
), asperpyrones A (
4
) and dianhydroaurasperone C (
5
) were identified using modern spectroscopy techniques such as MS and 1D, 2D NMR, and comparing with literature data. The single crystal of compound
2
was successfully obtained by culturing, whose axial chirality was further confirmed to
S
by single-crystal X-Ray diffraction experiment using Cu K
α
radiation. By comparing the CD spectra, it was determined that the axial chirality of compounds
1
-
5
were
S
.
红树林内生真菌Aspergillus sp.次级代谢产物二聚呫吨
mangroveendophytic fungusAspergillus sp.secondary metabolitedimer xanthones
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