基于网络药理学分析“黄芪―白术”治疗肺癌的作用机制

刘梓燊; 杨国旺

doi:10.13471/j.cnki.acta.snus.2020.04.26.2020E012

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基于网络药理学分析“黄芪―白术”治疗肺癌的作用机制

研究论文 | 更新时间：2023-11-01

- 基于网络药理学分析“黄芪―白术”治疗肺癌的作用机制
- Mechanisms of “Astragalus membranaceus - Atractylodes macrocephala koidz” for the treatment of lung cancer based on network pharmacology
- 中山大学学报(自然科学版) 2020年59卷第5期页码：134-143
- 作者机构：
  
  1.首都医科大学附属北京中医医院肿瘤科，北京 0000
  2.首都医科大学，北京 100069
- 作者简介：
  
  刘梓燊（1995年生），女；研究方向：中西医结合肿瘤；E-mail:liuzishen1001@163.com
  杨国旺（1969年生），男；研究方向：中西医结合肿瘤；E-mail:guowangyang@sina.com
- 基金信息：
  
  国家“十三五”重大新药创制专项课题(2019ZX09734001);国家重点研发计划项目(2018YFC1705101);2019年重大疑难疾病中西医临床协作能力建设项目（肝癌）
- DOI：10.13471/j.cnki.acta.snus.2020.04.26.2020E012
  中图分类号： R273
- 纸质出版日期：2020-09-25，
  
  收稿日期：2020-04-26，
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刘梓燊,杨国旺.基于网络药理学分析“黄芪―白术”治疗肺癌的作用机制[J].中山大学学报(自然科学版),2020,59(05):134-143.

LIU Zishen,YANG Guowang.Mechanisms of “Astragalus membranaceus - Atractylodes macrocephala koidz” for the treatment of lung cancer based on network pharmacology[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(05):134-143.
刘梓燊,杨国旺.基于网络药理学分析“黄芪―白术”治疗肺癌的作用机制[J].中山大学学报(自然科学版),2020,59(05):134-143. DOI： 10.13471/j.cnki.acta.snus.2020.04.26.2020E012.

LIU Zishen,YANG Guowang.Mechanisms of “Astragalus membranaceus - Atractylodes macrocephala koidz” for the treatment of lung cancer based on network pharmacology[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(05):134-143. DOI： 10.13471/j.cnki.acta.snus.2020.04.26.2020E012.

摘要

通过网络药理学的研究方法，探索“黄芪―白术”治疗肺癌的可能作用机制。使用中药系统药理学分析平台（TCMSP）数据库查询“黄芪―白术”的活性成分，运用TCMSP数据库对活性成分的靶点进行筛选，并利用Uniprot数据库对靶点进行标准化命名。通过“TTD”数据库、“DisGeNET”数据库和“OMIM”数据库查询肺癌相关靶点。利用Venn Diagram得到“化合物―疾病”共同靶点。运用STRING数据库进行蛋白质互作网络（PPI）的构建。使用R语言进行GO富集分析和KEGG通路富集分析。“黄芪―白术”药对共筛选得到活性成分19个，潜在作用靶点180个。治疗肺癌的预测靶点29个，主要涉及AKT1、VEGFA、EGFR等。GO功能富集分析，按照

＜0.05进行筛选，涉及生物过程1 260个，细胞组分12个，分子功能32个，主要涉及活性氧代谢过程、类固醇激素反应、凋亡信号通路的调控、T细胞活化等。KEGG信号通路富集分析，按照

＜0.05进行筛选，涉及信号通路26个，主要涉及PI3K-Akt信号通路、EGFR酪氨酸激酶抑制剂耐药性、铂类耐药性、HIF-1信号通路、MAPK信号通路、Rap1信号通路、Ras信号通路等。“黄芪―白术”通过调控信号通路、抗癌药物耐药性等发挥抑制肿瘤血管生成、诱导肿瘤细胞凋亡、抑制肿瘤转移等作用，从而治疗肺癌。“黄芪―白术”在治疗肺癌的过程中涉及到多活性成分、多靶点及多信号通路。

Abstract

The study aimed to explore the possible mechanism of “

Astragalus membranaceus - Atractylodes macrocephala

koidz” for the treatment of lung cancer through network pharmacology．The active ingredients of “

Astragalus membranaceus - Atractylodes macrocephala

koidz ” were queried through the TCMSP database

the targets of the active ingredients were screened through the TCMSP database

and the targets were standardized and named through the Uniprot database. The lung cancer related targets were queried through the “TTD” database

“DisGeNET” database and “OMIM” database. The “compound-disease” common targets were obtained using Venn Diagram

and the protein interaction network (PPI) was constructed using STRING database

and R language was used for GO enrichment analysis and KEGG pathway enrichment analysis.“

Astragalus membranaceus-Atractylodes macrocephala

koidz” drugs were screened to obtain 19 active ingredients

180 potential targets

29 potential targets for the treatment of lung cancer

including AKT1

VEGFA

EDFR

etc. GO functional enrichment was analyzed and screened according to

0.05

which involved 1 260 biological processes

12 cell groups

32 molecular functions

such as reactive oxygen species metabolic process

response to steroid hormone

regulation of apoptotic signaling pathway

T cell activation

etc. KEGG signal pathway enrichment was analyzed and screened according to

0.05

involving 26 signal pathways

including PI3K-Akt signaling pathway

EGFR tyrosine kinase inhibitor resistance

Platinum drug resistance

HIF-1 signaling pathway

MAPK signaling pathway

Cellular senescence

Rap1 signaling pathway

Ras signaling pathway

etc."

Astragalus membranaceus - Atractylodes macrocephala

koidz " plays a role in inhibiting tumor angiogenesis

inducing tumor cell apoptosis and inhibiting tumor metastasis by regulating signaling pathways and anti-cancer drug resistance

thereby treating lung cancer. “

Astragalus membranaceus-Atractylodes macrocephala

koidz” involves multiple active ingredients

multiple targets and multiple signaling pathways in the treatment of lung cancer.

关键词

网络药理学肺癌黄芪―白术作用机制

Keywords

network pharmacologylung cancerAstragalus membranaceus - Atractylodes macrocephala koidzmechanism of action

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