1.中国水产科学研究院南海水产研究所 / 热带水产研究开发中心 / 三亚热带水产研究院, 海南 三亚 572018
2.农业农村部南海渔业资源开发利用重点试验室,广东 广州 510300
赵旺(1987年生),男;研究方向:甲壳类贝类繁殖生物学;E-mail: zhaowang522@163.com
温为庚(1963年生),男;研究方向:甲壳类繁殖生物学;E-mail: wenweigeng@163.com
纸质出版日期:2021-07-25,
网络出版日期:2020-10-28,
收稿日期:2020-04-20,
录用日期:2020-06-05
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赵旺,温为庚,谭春明等.饥饿胁迫对猛虾蛄不同组织免疫酶活性的影响[J].中山大学学报(自然科学版),2021,60(04):26-33.
ZHAO Wang,WEN Weigeng,TAN Chunming,et al.Effects of starvation stress on the activities of immune enzymes in different tissues of Harpiosquilla harpax[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2021,60(04):26-33.
赵旺,温为庚,谭春明等.饥饿胁迫对猛虾蛄不同组织免疫酶活性的影响[J].中山大学学报(自然科学版),2021,60(04):26-33. DOI: 10.13471/j.cnki.acta.snus.2020.04.20.2020E010.
ZHAO Wang,WEN Weigeng,TAN Chunming,et al.Effects of starvation stress on the activities of immune enzymes in different tissues of Harpiosquilla harpax[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2021,60(04):26-33. DOI: 10.13471/j.cnki.acta.snus.2020.04.20.2020E010.
研究了饥饿胁迫对猛虾蛄
Harpiosquilla harpax
肠道、血液、肝胰脏和肌肉的六种免疫酶活性的影响。实验虾蛄禁食1、6、11和21 d后,分别采集样品并测定酶活力。结果表明:饥饿胁迫时间对猛虾蛄免疫酶活性影响显著(
P
<
0.05)。轻度饥饿时猛虾蛄免疫酶活性被诱导,重度时则被抑制。随着饥饿时间的延长:1)肠道中各免疫酶(过氧化氢酶(CAT)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)和过氧化物酶(POD))的活力均呈现“先增后降”的趋势;2)血液中除了CAT呈现“先降后增”外,其他为“先增后降”; 3)肝胰腺中CAT活力逐渐下降,而GSH-PX却“先降后增”,其他“先增后降”;4)肌肉组织中AKP略微下降,其他为“先增后降”。
This study was conducted to investigate the effects of starvation stress on the activities of six immune enzymes in the intestine, blood, hepatopancreas and muscle of
Harpiosquilla harpax
. The experimental animals were starved for 1, 6, 11 and 21 days, then samples were collected and activities of immune enzyme were analyzed, respectively. The results showed that, effect on the activities of immune enzymes was significantly by starveling time (
P
<
0.05). The immune enzyme activity of the animal was induced when mild starvation and inhibited under severe stress. With the prolongation of starveling time, it was shown that: 1) the activities of various immune enzymes of CAT, ACP, AKP, T-SOD, GSH-PX and POD in the intestine all showed a trend of "increase first, then decrease"; 2) in blood, except CAT, showed a trend of "first decrease then increase", the others increased first and then decreased; 3) in hepatopancreas, the activity of CAT decreased gradually, ACP, AKP, T-SOD and POD showed a trend of "increase first and then decrease", while GSH-Px showed a trend of "decrease first and then increase"; 4) in muscle tissue, except that AKP slightly decreased, the other immune enzyme activities showed a trend of "increase first and then decrease".
猛虾蛄Harpiosquilla harpax饥饿胁迫免疫酶抗氧化
Harpiosquilla harpaxstarvation stressimmune enzymeantioxidant
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