澳洲宝石鲈热休克蛋白90-β基因（<italic style="font-style: italic">HSP90-β</italic>）的cDNA克隆与表达

高凇泽; 孙彩云; 吴金英; 叶航宇; 李文笙

doi:10.13471/j.cnki.acta.snus.2019.10.18.2019E026

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澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达

研究论文 | 更新时间：2023-11-01

- 澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达
- Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)
- 中山大学学报(自然科学版) 2020年59卷第4期页码：7-18
- 作者机构：
  
  中山大学生命科学学院/水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室/ 广东省重要经济鱼类健康养殖工程技术研究中心，广东广州 510275
- 作者简介：
  
  高凇泽（1993年生），男；研究方向：鱼类生理和鱼类免疫学；E-mail: gaosz@mail2.sysu.edu.cn
  吴金英（1963年生），女；研究方向：鱼类生理和鱼类免疫学；E-mail: lsswjy@mail.sysu.edu.cn
- 基金信息：
  
  现代农业产业技术体系专项资金项目;国家特色鱼淡水鱼产业技术体系(CARS-46);现代农业人才支撑计划项目(2016-2020)
- DOI：10.13471/j.cnki.acta.snus.2019.10.18.2019E026
  中图分类号： Q17
- 纸质出版日期：2020-07-20，
  
  收稿日期：2019-10-29，
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高凇泽,孙彩云,吴金英等.澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达[J].中山大学学报(自然科学版),2020,59(04):7-18.

GAO Songze,SUN Caiyun,WU Jinying,et al.Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(04):7-18.
高凇泽,孙彩云,吴金英等.澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达[J].中山大学学报(自然科学版),2020,59(04):7-18. DOI： 10.13471/j.cnki.acta.snus.2019.10.18.2019E026.

GAO Songze,SUN Caiyun,WU Jinying,et al.Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(04):7-18. DOI： 10.13471/j.cnki.acta.snus.2019.10.18.2019E026.

摘要

热休克蛋白（heat shock proteins，HSPs）在鱼类的应激与免疫反应中起着重要的作用，HSP90-β是该家族的重要成员。为了探讨澳洲宝石鲈（

Scortum barcoo

）HSP90-β在免疫应答中的作用，本研究首次克隆得到了澳洲宝石鲈

HSP90-β

的全长cDNA序列。该序列长2 708 bp，其中5' UTR长86 bp，3' UTR长444 bp，ORF长2 178 bp，编码725个氨基酸，相对分子质量约为83 200，具有5个HSP90家族的特征序列，没有信号肽序列和跨膜结构域，与其他鱼类HSP90-β氨基酸的序列一致性皆超过90%。正常情况下，澳洲宝石鲈

HSP90-β

mRNA主要在肝脏表达。而感染无乳链球菌(

Streptococcus agalactiae

)后，其在肝组织中的表达呈先降低后增加的趋势，在脾脏、头肾和脑组织则呈先上升后下降的趋势。这些结果表明澳洲宝石鲈HSP90-β可能参与了无乳链球菌感染引发的免疫反应。

Abstract

Heat shock proteins (HSPs) play important roles in fish stress and immune response

and HSP90-β is an important member of the HSPs family. In the present study

to investigate the possible role of HSP90-β in the immune response

the full-length cDNA sequence of

HSP90-β

was first cloned from jade perch (

Scortum barcoo

). The size of the full-length cDNA of jade perch

HSP90-β

is 2 708 bp

of which 5' UTR is 86 bp

3' UTR is 444 bp

and ORF is 2 178 bp

which encodes 725 amino acids. The molecular weight of jade perch HSP90-β is predicted as 83 200. And this protein owns five characteristic sequences of the HSP90 family. Moreover

it has no signal peptide and transmembrane domain. Phylogenetic analysis showed that the amino acid sequence identity of jade perch HSP90-β is more than 90% with those of other fishes. Under normal circumstances

the

HSP90-β

mRNA of jade perch is mainly expressed in the liver. After infection with

Streptococcus agalactiae

the

HSP90-β

mRNA expression decreased first and then increased in liver. Differently

it increased first and then decreased in spleen

head kidney and brain. This result suggests that the HSP90-β may be involved in the immune response elicited by

S. agalactiae

infection in jade perch.

关键词

澳洲宝石鲈HSP90-β分子克隆表达

Keywords

Scortum barcooHSP90-βmolecular cloningexpression analysis

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澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达

Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)

DOI：10.13471/j.cnki.acta.snus.2019.10.18.2019E026

摘要

Abstract

关键词

Keywords

references