When there are many unreachable paths in the road network, there exists mutual influence between the paths, and the optimization cannot reach the overall optimum. Aiming at this phenomenon, a new integrated optimization model is presented. Firstly, the accessibility and comprehensive optimization of guide path are analyzed. Based on this, a comprehensive path optimization guidelines model is proposed with the optimization of the path length and the number of guidance information. Then combining with artificial bee colony algorithm and a neighborhood search strategy for road networks, the algorithm is designed to solve the problem of the concrete design. Finally, Guangzhou Higher Education Mega Center is chosen as the test area. The integrated optimization guidelines of the multiple guidance paths with the aim of Sun Yat-sen University are realized using the proposed model. In addition, comparing with single optimization results, experimental results show that the model can effectively solve the optimization problem for multiple path guidance, and the path quality is consider as a whole.