In this study

DNA  cytosine methylation modification level and MSAP amplification graphs of Lycoris longituba were obtained from seed embryo

callus and seedling via methylation sensitive amplification polymorphism technology. We found that 51 pairs of MSAP selective amplifying primer combinations were good in 64 pairs of the MSAP selective amplifying primer combinations.The results showed that the cytosine methylation rates were 68.0% in seed embryo (full methylation:50.2% and half methylation:49.8%)

60.0% in callus(full methylation:52.2% and half methylation:47.8%) and 64.3% in seeding(full methylation:40.8% and half methylation:59.2%).The level of methylation differed with each other in the three different statges. So

it is suggested that changes of the level of methylation may had led to their different organs of Lycoris longituba based on the modification mechanism of super methylation/demethylation analysis.