Random amplified polymorphic DNA(RAPD) is a new molecular marker technology developed by Williams and Welsh in 1990.Sixteen mustard( Brassica juncea Coss.)cultivars of eight varieties were identified with RAPD markers. Seven 10 mer arbitrary primers were selected out of sixty one

and genomic DNA fingerprints of sixteen mustard cultivars were obtained. One or two characteristic DNA bands were shared by each primer among sixteen cultivars. RAPD markers between two mustard cultivars of one variety were rich and two cultivars could be distinguished through comparing the different DNA bands between them. No single primer out of seven could distinguish all the eight pair cultivars on one analysis while combination of two or more primers could do it. The results showed that identification of mustard cultivars with RAPD markers is a sensitive and rapid approach technology which can be applied widely in seed scientific researches and seed production.