The transgenic Del gene tobacco plants were obtained by co cultivation with Agribacterium tumefacience LBA4404 (pJAM 175). Successful transformation of NPT Ⅱgene to tobacco plants of T 0 generation was demonstrated by PCR Southern blot hybridization in 8 of 9 putative transgenic plants. And then

successful transformation of Del cDNA to tobacco plants of T 0 generation was also demonstrated by PCR Southern blot hybridization in all of 5 flowering plants carrying NPT Ⅱ gene. Furthermore

the assay of PCR Southern blot hybridization demonstrated the integration of Del gene in the genome of kanamycin resistant plants of T 1 generation. The absorbance of the extracts from the transgenic plants of T 0 generation was measured at 530 nm. In 3 transgenic plants

pigment extractions from sepals

petals and stamens all accumulated more anthocyanin than the control plants. But carpels and leaves did less. To some extent

the results showed the biological functions of Del gene and demonstrated and supplemented the conclusion on pigmentation pattern mode by Goodrich et al.