The sequence of internal transcribed spacer(ITS) regions of nuclear ribosomal DNA (nrDNA) was determined for the endemic species Siphocranion nudipes (Hemsl ) Kudo (Lamiaceae) from China. This is the first sequence report of the ITS regions for Lamiaceae. The entire ITS and 5 8S regions of Siphocranion nudipes are 609 bases long with an ITS 1 of 207 bp
an ITS 2 of 236 bp and a 5 8S of 166 bp. The overall G+C content was 59 11% with 64 25% in ITS 1
57 63% in ITS 2
and 53 61% in 5 8S.The numerous copies of the ITS sequences within an individual in Siphocranion nudipes showed a high level of sequence homogeneity and only three nucleotide positions were ambiguous. This sequence will serve as a basis for determining the phylogenetic status of Siphocranion nudipes within Lamiaceae and for indepth studies on the conservation of this endemic species from subtropical areas of China.
关键词
&nbsp序列光柄筒冠花ITS区
Keywords
&nbspsequenceSiphocranion nudipesinternal transcribed spacer regions