When immature cotyledons of C. ptilophylla were cultured on SH medium supplemented with 0 5mg·L -1 2

4 D or 0 5 mg·L -1 2

4 D and 2 mg·L -1 PCPA

somatic embryos could be induced from hypocotyls of zygotic embryos after two weeks of culture

and after 5￣7 weeks somatic embryos could be induced directly from the epidermis of cotyledons. Embryos could be induced after only 4 weeks of culture and the induced frequency was improved by reducing the content of 2

4 D to 0 2 mg·L -1 . If these cultures were transferred to SH medium supplemented with 2 mg·L -1 BA and 0 2 mg·L -1 IBA after 15 weeks

“seed like embryos”

“bud like embryos” and “cup shape embryos” were induced and plantlet could be regenerated from these somatic embryos in the medium. “Caespitose buds” could also be induced and developed into shoots in this medium. If “bud like embryos” were transferred to medium with 5 mg·L -1 GA 3 and 1 mg·L -1 IAA

they developed into shoots without root with higher frequency.