Using the sandwich enzyme linked immunosorbent assay(ELISA) for grass carp(Ctenopharyngodon idellas) growth hormone developed

kinetics of rcGH in crucian carp and jian carp blood after administrations of rcGH were investigated. Serum GH levels in crucian carp following an intraperitoneal(i p ) injection of rcGH (1 μg/g) increased quickly

reaching maximum after 2 h

didn’t return to normal level after 8 d

whereas serum GH levels post an i p injection of rcGH ( 0.2 μg/g ) to crucian carp increased slowly

reaching a maximum post 6 h and returned to normal levels after 8 d. Following 3 i p injections of rcGH (0.2

1

5 μg/g) to crucian carp simultaneously

serum GH levels in 0.2 μg/g group returned to normal levels after 2 d

but serum GH levels in 1 and 5 μg/g group didn’t still return to normal values after 8 d. Serum GH levels post 3 i p injections of rcGH (0.5

1

5 μg/g) to jian carp at the same time returned to normal values after 3

5 and 7 d postinjection

respectively.