Retroviral recombinant pLX(hIL 10)SN was constructed and introduced it into packaging cell line PA317.After PA317 was selected by G418

using recombinant retrtoviral vector pLX(hIL 10)SN to infect rat glomerular mesangial cells.After gene transfer 72 h

DNA and RNA were prepared from rat transfected mesangial cells for the polymerase chain reaction(PCR) and the reverse transcription polyverase chaim reaction(RT PCR).Exogenous of hIL 10gene has been subcloned into retrovial vector pLXSN successfully

and it has been integrated into the chromosal DNA of the transfected masangial cells.RT PCR and ELISA showed that exogenous hIL 10 gene was experssed in the transtected mesangial cells after transfection 48 h and lasting for over 28 d.The retroviral vector pLX(hIL 10)SN shall lay foundation for researching hIL 10 mechanism and its application for curing deseases.