1. 中国水产科学研究院淡水渔业研究中心,农业部水生动物遗传育种和养殖生物学重点开放实验室,江苏,无锡,214081
2. 江阴市水产指导站,江苏 江阴,214075
纸质出版日期:2010,
网络出版日期:2010-1-25,
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徐钢春, 张呈祥, 顾若波, 等. 似刺鳊鮈精子的超显微结构及主要生物学特性[J]. 中山大学学报(自然科学版)(中英文), 2010,49(1):80-84.
XU Gangchun, ZHANG Chengxiang, GU Ruobo, et al. Spermatozoal Ultrastructure and the Main Biological Characteristics of Paracanthobrama guichenoti[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2010,49(1):80-84.
徐钢春, 张呈祥, 顾若波, 等. 似刺鳊鮈精子的超显微结构及主要生物学特性[J]. 中山大学学报(自然科学版)(中英文), 2010,49(1):80-84. DOI:
XU Gangchun, ZHANG Chengxiang, GU Ruobo, et al. Spermatozoal Ultrastructure and the Main Biological Characteristics of Paracanthobrama guichenoti[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2010,49(1):80-84. DOI:
选取健壮无伤的似刺鳊鮈亲鱼进行人工采精,应用扫描电镜和透射电镜观察了其精子超微结构,同时研究了不同温度、盐度对精子活力的影响。结果显示:① 似刺鳊鮈精子由头部、中片和鞭毛组成。头部近圆形,主要结构是细胞核,核前端无顶体,核中染色质致密均匀,后端有植入窝;中片的袖套与细胞核相连,含有丰富的线粒体和囊泡;尾部细长,主要结构是轴丝,为典型的“9+2”微管结构。② 似刺鳊鮈精子活力较适宜温度为20~28 ℃,温度为24 ℃时精子寿命最长,为1 900 s;较适盐度为5‰~8‰,盐度7‰时精子寿命最长,为1 350 s。
The healthy male Paracanthobrama guichenoti were employed and the spermatozoa were collected from the body by artificial conduction. The sperm was observed under the scan electron microscope. Meanwhile
effects of temperature
salinity on sperm motility were studied. The results showed that: the sperm of Paracanthobrama guichenoti containing 3 parts
a head
a middlepiece and a long tail. The head is subcircle in shape. The main structure of the head is nucleus. There is no acrosome at the anterior end of the nucleus
whereas there is an implantation fossa at the posterior end of the nucleus
within which the chromatin is dense; The sleeve of the middlepiece
which is rich in mitochondria and vesicles
is connected to the posterior end of the nucleus; The tail part is slender
including principal piece and end piece. The main structure is axoneme
which is the structure of a typical “9+2”.The effects of water temperature on spermatozoa motility showed that the optimum water temperature is 24 ℃ when the spermatozoa have the longest life of span(1 900),and the optimum range of water temperature is 20~28℃. When the salinity of water at 5‰~8‰,the spermatozoa have the best motility and the spermatozoa have the longest life span at salinity 7‰,which is 1 350 s.
似刺鳊鮈精子微结构生物学特性
Paracanthobrama guichenotispermultrastructurebiological characteristic
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