The chemical constituents of Vernonia spirei Gandog were studied. The constituents were separated and purified from MeOH extract of the whole plant with column chromatographic methods through silica gel

RP18 silica gel

Sephadex LH20

HPLC

and preparative TLC. Their chemical structures were characterized by spectroscopic methods including NMR and EIMS. The result showed that Vernonia spirei Gandog contained two components including 5

7

4〖DK〗’trihydroxy6

3 ’dimethoxyflavone(Ⅰ)

5

3 ’dihydroxy6

7

4 ’trimethoxyflavone(Ⅱ). These compounds were obtained from this genus for the first time.