Traditional chinese medicine of Ilex pubescens (Mao-Dong-Qing) is commonly used to treat the cardiovascular diseases in South China. The preliminary bioassays showed that the total saponins isolated from Ilex pubescens exhibited significant potent degrading the plasma viscosity in rats. Meanwhile

our recent works indicated that the n-BuOH soluble fractions of ethanol extract (70%) from the root of the same species obviously inhibited the contraction (60 μg/mL) of rabbit thoracic aorta induced by norpinephrine in vitro. As a continuation of bioactive constituent from the n-BuOH of Ilex pubescens

five triterpene saponins were isolated. Their structures were elucidated by chemical

physical methods and spectroscopic analysis methods. As a result

they were identified as mussaendoside R (1)

ziyu-glycoide I (2)

Lucyoside H (3)

ilexoside A (4)

ilexoside O (5)

excepescially. Compounds 1

2 and 3 were firstly obtained from Ilex pubescens.