斜带石斑鱼转化生长因子β1（TGFβ1）基因的克隆及表达分析

平海林; 吴金英; 徐胜威; 胡开顺; 段志刚

您当前的位置：

首页 >

文章列表页 >

斜带石斑鱼转化生长因子β1（TGFβ1）基因的克隆及表达分析

研究论文 | 更新时间：2023-12-11

- 斜带石斑鱼转化生长因子β1（TGFβ1）基因的克隆及表达分析
- Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides)
- 中山大学学报(自然科学版)(中英文) 2011年50卷第1期页码：92-98
- 作者机构：
  
  中山大学生命科学学院水生经济动物研究所∥广东省水生经济动物良种繁育重点实验室,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-1-25，
扫描看全文
平海林, 吴金英, 徐胜威, 等. 斜带石斑鱼转化生长因子β1（TGFβ1）基因的克隆及表达分析[J]. 中山大学学报(自然科学版)(中英文), 2011,50(1):92-98.

PING Hailin, WU Jinying, XU Shengwei, et al. Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides)[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2011,50(1):92-98.
平海林, 吴金英, 徐胜威, 等. 斜带石斑鱼转化生长因子β1（TGFβ1）基因的克隆及表达分析[J]. 中山大学学报(自然科学版)(中英文), 2011,50(1):92-98. DOI：

PING Hailin, WU Jinying, XU Shengwei, et al. Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides)[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2011,50(1):92-98. DOI：

摘要

转化生长因子β1（TGFβ1）是一种高度保守的多效细胞因子。通过分子克隆得到了斜带石斑鱼Epinephelus coioidesTGFβ1 cDNA全长序列，全长为2 477 bp，开放阅读框为1 161 bp，编码一种含386个氨基酸的多肽。Realtime PCR分析表明，TGFβ1基因在健康斜带石斑鱼所取组织中均有表达，肾脏中表达最高，其次是头肾和脾脏。用PolyI:C或ConA刺激斜带石斑鱼头肾淋巴细胞，TGFβ1表达量明显上升，且在刺激4 h后达到最高值。用PolyI:C刺激16、24 h后，TGFβ1几乎不表达，而用ConA刺激实验组，TGFβ1表达量一直高于对照组。结果说明，斜带石斑鱼TGFβ1基因结构和表达特征与其他硬骨鱼类具有高度相似性。

Abstract

Transforming growth factorβ1 (TGFβ1) is a highly conserved pleiotropic cytokine. In this study

we presented the bioinformatics analysis of TGFβ1 fulllength cDNA sequences from orangespotted grouper (Epinephelus coioides). The full length cDNA is 2477 bp

which contains an ORF of 1161 bp

encoding a 386 amino acids protein. Realtime PCR results showed that TGFβ1 mRNA was predominantly expressed in the kidney

followed by head kidney and spleen of the healthy orangespotted grouper. The expression of TGFβ1 in isolated headkidney lymphocytes was upregulated treated with PolyI:C or ConA

and peaked at 4 hours after stimulation. Lymphocytes groups which were treated with PolyI:C had little TGFβ1 expression after 16 and 24 hours

while the expression of TGFβ1 was significantly higher than the control groups during the whole experimental processing time treated with ConA. In conclusion

we found the structure and expression pattern of grouper TGFβ1 was similar to those of other fish.

关键词

TGFβ1斜带石斑鱼Epinephelus coioides克隆表达分析

Keywords

TGFβ1Epinephelus coioidescloningexpression

references

浏览量

126

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

水稻几丁质酶基因(Oschi)cDNA的克隆及序列分析

牙鲆fibrinogen β基因的克隆及表达分析

异色瓢虫的HSP 90基因的克隆与特性分析

斑马鱼3种胰蛋白酶原基因的克隆、序列分析及组织表达

橘小实蝇半胱氨酸蛋白酶基因克隆及表达模式