To observe the brain image and the change of Ca

2+

 level in live mouse by using twophoton laser scanning confocal microscope. Using craniotomy to expose target area of mouse brain(somatosensory cortex) then injectCa

2+

sensitive fluorescent indicator

for instance

Oregon Green 488 BAPTA-1 acetoxymethyl. After that

depose the mouse under the twophoton to observe and record. The brain image record by twophoton is clear and vivid .And photograph indicate that by using twophoton confocal can reach deeper area of the brain and record the fast change of Ca

2+

 in single neuron. The results show that by using multiphoton confocal microscope couple with calcium sensitive fluorescent indicator can get high resolution image of brain of live mouse.