CHENG Yue, FU Ying, WANG Zhiyu, et al. Determination on the Contents of Condensed Tannins in Spatholobus suberectus Dunn.Extracts and Primary Study on their Anti-tumor Activities[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2011,50(2):75-80.
CHENG Yue, FU Ying, WANG Zhiyu, et al. Determination on the Contents of Condensed Tannins in Spatholobus suberectus Dunn.Extracts and Primary Study on their Anti-tumor Activities[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2011,50(2):75-80.DOI:
The contents of total flavonoids and condensed tannins in the ethanol extract of
Spatholobus suberectus
Dunn and its different solvents partitioned extracts were determined by ultraviolet spectrophotometry and VanillinH
2
SO
4
method
respectively. The contents of total flavonoids in the φ=60% ethanol extract
ethyl acetate insoluble extract
ethyl acetate partitioned extract
n-butanol partitioned extract
water residue were (6.49±1.99)%,(7.36±0.13)%,(9.35±0.22)%,(8.41±0.27)%,(4.48±1.53)%, respectively
while their contents of condensed tannins were (51.09±0.37)%,(52.34±4.73)%,(73.22±2.72)%,(83.11±5.87)%,(29.74±2.12)%
respectively. The inhibitory activities on the proliferation of HT-29 and MCF-7 tumor cells were determined by MTT. The extract samples all showed inhibitory activities in different extent on the proliferation of HT-29 and MCF-7 tumor cells. The nbutanol partitioned extract had the strongest inhibitory activities on the proliferation of both tumor cells.
关键词
鸡血藤缩合鞣质总黄酮抗肿瘤细胞增殖
Keywords
Spatholobus suberectusDunncondensed tanninstotal flavonoidsanti-proliferation of tumor cell