鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建

苏胜彦; 董在杰; 袁新华; 梁政远; 曲疆奇; 张建桥; 刘伟; 马良骁; 徐跑; 

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鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建

研究论文 | 更新时间：2023-12-11

- 鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建
- Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.)
- 中山大学学报(自然科学版)(中英文) 2012年51卷第2期页码：77-85
- 作者机构：
  
  1. 南京农业大学无锡渔业学院,江苏,无锡,214081
  2. 
  3. 中国水产科学研究院淡水渔业研究中心∥农业部淡水渔业和种质资源利用重点实验室，江苏,无锡,214081
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-3-25，
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苏胜彦, 董在杰, 袁新华, 等. 鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建[J]. 中山大学学报(自然科学版)(中英文), 2012,51(2):77-85.

SU Shengyan, DONG Zaijie, , et al. Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.)[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2012,51(2):77-85.
苏胜彦, 董在杰, 袁新华, 等. 鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建[J]. 中山大学学报(自然科学版)(中英文), 2012,51(2):77-85. DOI：

SU Shengyan, DONG Zaijie, , et al. Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.)[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2012,51(2):77-85. DOI：

摘要

为了了解鲤

IGF2b

IGF2

IGF2b

IGF2b-

IGF2b

Abstract

To explore the relationship between the

IGF2b

gene and growth traits

we cloned the introns of

IGF2b

in common carp (Cyprinus carpio ) muscle and analysis its characters. Further more

the lentiviral vector(Lenti-

IGF2-

IRES-EGF) was constructed and its activity was also researched. The results showed that: the length of 5 173 bp DNA sequence of 

IGF2b

(HM755899) was get

including 3 introns and 4 exons. Two CpG islands in the 3′untranslated region and (T)n repeat sequence in the 5′untranslated region were found. Besides these

the lentiviral vector(Lenti-

IGF2b-

IRES-EGF) was constructed successfully

which was cotransfected into 293T cells with the GFP expression. The higher expression of IGF2 was found in 293T cells by Real time PCR. These illustrated that these study may be lay on solid foundation for relationship between

IGF2b

genetic diversity

expression and common carp growth traits.

关键词

鲤IGF2b内含子慢病毒载体

Keywords

common carpIGF2bintronslentiviral vector

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