the HPLC fingerprint of Kouyanqing Granule was established with Dikma PLATISIL ODS (250 mm×4.6 mm
5 μm) column
acetonitrile and 0.1% (φ) formic acid as mobile phase
and the detection wavelength was 254 nm. 23 common peaks were selected as the fingerprint peaks. Seven of these common peaks were identified as chlorogenic acid
caffeic acid
luteolin-7-O-glucoside
harpagoside
cinnamic acid
ammonium glycyrrhetate and glycyrrhizic acid. The method established is selective
simple and reproducible
which can be used for the quality control of Kouyanqing Granule.