Use of antibiotics at subtherapeutic concentrations for agricultural applications has been believed to be an important factor in the proliferation of bacteria with antibiotic resistance genes (ARGs). In this work

total nine tetracycline ARGs (tet-ARGs) including tetA

tetB

tetC

tetD

tetG

tetL

tetO

tetQ and tetX) were studied in sixteen soil samples from livestock using qualitative PCR SYBR Green I real-time PCR methods. The results showed that eight of tet-ARGs except tetD could be detected in most of samples and the detection percentages ranged from 6% (tetB) to 94% (tetC). The quantitative results for four tet-genes (tetA

tetC

tetG and tetX) showed that 50% samples had the highest tetC abundance (10

8

) and the others were in range of 10

2

to 10

5

. As a result

the spread occurrences of high levels of tet-genes in livestock soil should not be overlooked.