LIANG Cuiyi, XU Jingliang, YUAN Zhenhong, et al. Application Studies on High Activity β-Glucosidase Producing Strain[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2013,52(1):118-122.
LIANG Cuiyi, XU Jingliang, YUAN Zhenhong, et al. Application Studies on High Activity β-Glucosidase Producing Strain[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2013,52(1):118-122.DOI:
Studies of β-glucosidase enzymatic properties from
Hypocrea
sp. W
63
show that
enzyme activity determined by pNPG (4-nitrophenyl β-D-glucopyranoside) can attain to 482.1 U/mL. The optimum reaction pH value and temperature are 4.8 and 65 ℃
respectively. 10% of ethanol has the greatest promotion
the β-glucosidase activity can nearly increased double and the ethanol tolerance of β-glucosidase can attain to 30%. When simultaneous saccharification and fermentation(SSF) carried out for 120 h with β-glucosidase adjunction
41.25 g/L of ethanol can be obtained. Compared with control
the ethanol production can increase nearly 2-fold. The β-glucosidase with high enzyme activity
thermostable and alcohol tolerance for SSF application
indicate that the β-glucosidase producing strain has great potent application on cellulosic ethanol production.