Isolation, Identification and Enzyme-producing Conditions Optimization of#br#
Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite
GAO Yunhang, WANG Wei, LI Qiuju, et al. Isolation, Identification and Enzyme-producing Conditions Optimization of#br#
Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2013,52(2):84-89.
GAO Yunhang, WANG Wei, LI Qiuju, et al. Isolation, Identification and Enzyme-producing Conditions Optimization of#br#
Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2013,52(2):84-89.DOI:
Five strains of bacteria with decomposed function for lignin and cellulose were isolated from gut of termite with a congo red and Azure-B plate method. MX5 strain with the strongest decomposed function was identified as Bacillus licheniformis by morphological observation
physiological and biochemical identification and 16S rRNA identification. Optimization test of producing lignin and cellulose show MX5 strain has the highest production activity with 0.5% of straw as carbon source
mixture of 0.5% yeast extract and ammonium sulfate as the nitrogen source and cultrue in shake flask at 37 ℃ for 96 h with 1%noculation amount and initial pH 8.0. Selecting excellent strains which produced ligninase and cellulase has far-reaching significance for improving the utilization of wood fiber feed