It is important to study the interaction of small molecules with silk peptide. In this paper

the interaction of reactive dark blue M2GE(RDBMG) with silk peptide was studied by means of multiple spectroscopy. The binding distance and binding ratio were calculated as 304 nm and 1∶〖KG-*2〗1

respectively. Additionally

the effects of foreign substances in dyeing system such as surfactants

ionic liquids

metal ions

oxidants

textile pulp

and seven kinds of amino acids (histidine

 L-aspartic acid

 L-lysine

 L-arginine

 L-methylthio amino acid

 L-leucine and L-proline) on SP RDBMG were also investigated. The results showed that the binding constants increased when Ionic liquids including chloride hydroxyethyl trimethyl amine

bromide 1-ethyl-2

3–dimethyl imidazolium

1-ethyl-2

3-methyl imidazolium bis-［trifluoromethyl-sulfonyl］ imide

textile pulp (original starch

phosphate starch

oxidized starch)

metal ions (Ca

2 +



Zn

2 +



Ba

2 +

)

surfactants(CTAB

Triton X-100

sodium dodecyl sulfate) and seven types of amino acids were added into SPRDBMG. However

the binding constants decreased when polypropylene aldehyde amine

aldehyde ester starch

Cu

2 +

 and oxidants (φ=30%H

2

O

2

K

2

S

2

O

8

K

2

Cr

2

O

7

) were in the presence of SP-RDBMG.