Genetic diversity of three wild populations of 〖WTBX〗Siniperca kneri〖WTBZ〗 from Youjiang River，Zuojiang River and Red Water River  was studied by microsatellite DNA molecular markers. Total 19 pairs of highly polymorphic microsatellite primers of S. kneri

including 4 pairs exploited autonomously by bioinformatics and 15 pairs screened from 28 pairs of general primers

were adopted for this study. Analyzing 19 microsatellite locis for three populations

and total 102 individuals

Fixation Index among 3 populations varied from 0.001 4 to 1.000 0 showed the loss of heterozygosity

which leaded to lowering its response to the environment. Observed Number of Alleles (Na=19.473 7)

Shannons Information Index (I=2.399 8)

Average Heterozygosity (Ave_Het =0720 6)

and Polymorphism Information Content (0.447＜PIC＜0.947) were indicated the abundant genetic diversity among the three populations under Hardy-Weinberg equilibrium. But there was some differentiation among the three populations: Average Heterozygosity of Red Water River was the highest（Ave_Het=0.771 5）

and that of Zuojiang River and Youjiang River were lower and similar (0.692 1、0.698 3). The Genetic Differentiation Index (Fst) among 3 populations was 0.153 9

between 0.15 and 0.25

84.61% of which was contributed by intra-population and 15.39% by inter-population. Fis Value was 0.576 1. Gene Flow (Nm) of all locis were widely varied between 0.147 4 and 8.909 1

and its average was 0.795 7

which indicated there was the medium genetic differentiation level among populations

and gene flow was smooth in general. Their inbreeding coefficient was relatively high which changed easily genetic structure of population. The UPGMA dendrogram based on the Nei's genetic distance suggested that the populations of Zuojiang River and Youjiang River clustered firstly and then did with that of Red Water River. The dendrogram relationship and genetic distance of three populations are closely linked with geography distribution.