To continue to study anti-tumour bioactive saponins EtOAc layer from methanol extract of the stem of psychotria sp., four compounds were obtained by all kinds of column chromatography, such as silica gel, Sephdex LH-20, and ODS. Their structures were characterized by spectroscopy including NMR, MS, GCMS analyses, and chemical and the physical methods. The result indicated that they were Psychotrianosides G (1), Ardisianosides D (2),β-amyrin (3), and Stigmasterol3-O-β-D glycosidase(4). Cyclamiretin A (5) was obtained by acid hydrolysis of 2. Psychotrianosides G(1) was firstly obtained as a natural product. The chemical structure of compound (1) was analyzed by 2D NMR firstly. Compounds 1,2,3,4,5 were firstly obtained from genus of psychotria.