In this paper, the effect of various noises on the turbulence was discussed based on the field observation data in estuarine bottom boundary layer, and choosing three kinds of noise, the white Gaussian noise, the linear trend, and the periodic signal. The following results have been obtained: (1) The energy spectrum of the turbulence is more obviously affected than the probability distribution. The influence of white Gaussian noise on the energy spectrum is the largest, the influence of linear trend is not notable, and the periodic signal only makes effects on specific frequency. (2) White Gaussian noise and periodic signal both have influence on these three turbulent parameters: turbulent intensity, turbulent energy and turbulent energy dissipation rate, but the linear trend only has influence on turbulent intensity and turbulent energy. Adding noises to the turbulence will make the turbulent parameters increase.