Laser holography is an important method for fabricating photonic crystal microstructures. In order to facilitate adjusting beam parameters to improve the success rate of experiment, it is proposed to transfer the formed microstructure to a computer for real-time display and processing by using a CMOS sensor. According to the principle of multi-beam interference, the beam configurations and design principles of the realization of 2D microstructures are explored. Combining with numerical simulations, the influence of the beam polarizations on the shapes of the formed microstructure “atom” is also discussed. Further experiments validate the designs. With the help of the convenient real-time display and flexible adjusting of the beam parameters, two kinds of microstructures,i.e., the triangular and square lattices,are obtained. Experimental results show that the real-time computer display technology is helpful to improve the efficiency of design and fabrication of microstructures.