WANG Guohui, XU Yanan. The Fermentation Culture of VOCs Solubilization Microorganisms and Its Solubilization[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2015,54(5):85-89.
WANG Guohui, XU Yanan. The Fermentation Culture of VOCs Solubilization Microorganisms and Its Solubilization[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2015,54(5):85-89.DOI:
To improve the water-solubility and biodegradability of VOCs
VOCs solubilization microorganisms were domesticated to make its produce biosurfactant to make VOC solubilize. Benzene and toluene were selected as representative composition
such as solubilization activity and emulsifying rate as index
the fermentation conditions
production regularity and solubilization effect of microorganisms of VOCs solubilization etc were studied. The result showed that the solubilization microorganisms that produce BST were successfully domesticated. The optimum fermentation conditions for the production of BST was that the best carbon source concentration was 1.0 g/L and the optimal initial pH value was in the range of 6.95~7.05 and the optimum temperature range was in the range of 26~32 ℃. The solubilization bacteria had an obvious regularity for production of BST. The BST continue to increased rapidly during the fermentation of 0~36 h
and arrived at a maximum yield at 36 h. The fermentation liquid had good effect on the solubilization of benzene and xylene. The fermentation liquid had good effect on the solubilization of benzene and xylene. They were 2.7 times and 30 times of the pure medium
respectively. The emulsion droplets formed by benzene and BST were as an oil in water and it is soluble in water. This was more favorable for the biodegradation of VOCs. The bacteria producing BST were mostly gram negative bacilli. The BST was initially identified as a polysaccharide. The content of BST in the fermentation broth was about 60 g/L.