基于细菌/真菌相互作用的红树林内生真菌活性菌株筛选

张柳红; 李嫣然; 李云龙; 宁嘉盈; 周耀航; 熊智瑶; 李佳欣; 王玉如; 黄志坚; 刘岚

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基于细菌/真菌相互作用的红树林内生真菌活性菌株筛选

研究论文 | 更新时间：2023-12-11

- 基于细菌/真菌相互作用的红树林内生真菌活性菌株筛选
- Screening of antibacterial strains from mangrove endophytic fungi based on bacterial/fungal interactions
- 中山大学学报(自然科学版)(中英文) 2017年56卷第2期页码：93-101
- 作者机构：
  
  中山大学海洋科学学院,广东,广州,510006
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2017-3-25，
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张柳红, 李嫣然, 李云龙, 等. 基于细菌/真菌相互作用的红树林内生真菌活性菌株筛选[J]. 中山大学学报(自然科学版)(中英文), 2017,56(2):93-101.

ZHANG Liuhong, LI Yanran, LI Yunlong, et al. Screening of antibacterial strains from mangrove endophytic fungi based on bacterial/fungal interactions[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2017,56(2):93-101.
张柳红, 李嫣然, 李云龙, 等. 基于细菌/真菌相互作用的红树林内生真菌活性菌株筛选[J]. 中山大学学报(自然科学版)(中英文), 2017,56(2):93-101. DOI：

ZHANG Liuhong, LI Yanran, LI Yunlong, et al. Screening of antibacterial strains from mangrove endophytic fungi based on bacterial/fungal interactions[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2017,56(2):93-101. DOI：

摘要

为筛选出具有抑菌活性且次级代谢产物丰富的红树林内生真菌，以水产养殖中分离得到的2株病原细菌（Vibrio parahaemolyticus B1和Acinetobacter johnsonii B2）对2014年从珠海淇澳-担杆岛省级自然保护区采集得到的616株红树林内生真菌进行代谢产物抗菌活性和丰富度筛选。利用共培养技术，寻找在共培养后菌落形态特征出现明显变化或形成抑菌圈的内生真菌。通过对纯培养和共培养真菌的粗提物进行抑菌活性检测和HPLC指纹图谱分析，筛选出在共培养条件下可诱导产生新代谢产物且抑菌效果显著提高的菌株，为发现抗菌化合物提供新来源。

Abstract

In order to screen out mangrove endophytic fungal strains with antibacterial activity and abundant secondary metabolites

two strains of aquatic pathogenic bacteria named as Vibrio parahaemolyticus B1 and Acinetobacter johnsonii B2 were co-cultured with 616 strains of mangrove endophytic fungi

which were collected from Guangdong Zhuahai QiaoDangan island nature reserve in 2014

respectively. And then some of the endophytic fungi with obvious changes in the morphology of the colonies or produce inhibition zones after co-culture were investigated. The crude extracts of pure cultures and co-cultures were analyzed by HPLC fingerprints to find out some fungal strains that could induce new secondary metabolites and increase antibacterial effect in co-culture conditions. In addition

their antibacterial activity against V. parahaemolyticus B1 and A. johnsonii B2 were evaluated. It is a new strategy for these strains to produce novel antibacterial compounds.

关键词

红树林内生真菌抑菌活性HPLC指纹图谱次级代谢产物

Keywords

mangrove endophytic fungiantibacterial activityHPLC fingerprintsecondary metabolites

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