LIANG Ziqiang, LIANG Shike, LIU Tingting, et al. Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath)[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2017,56(1):121-124.
LIANG Ziqiang, LIANG Shike, LIU Tingting, et al. Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath)[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2017,56(1):121-124.DOI:
The long wing female adults of Sogatella furcifera were selected to extract their genomic DNA. According to the complete 18S rDNA gene sequence from Hemiptera
the unknown 18S rDNA sequence of S. furcifera was amplified by PCR. The complete 18S rDNA gene sequence of S. furcifera was obtained for the first time by splicing the unknown and known 18S rDNA sequences
whole sequence length was 1 891 bp. Comparing 18S rDNA sequence from S. furcifera and those from other insects
four conserved regions were found and the second region had the least insertion or deletion. Based on the second region
phylogenetic analysis was made and the results showed that most Homoptera insects used in this study had a close relatives with Eurydema maracandica (Hemiptera) except Delphacida.