补肺活血胶囊中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的测定方法

唐清; 朱晓枭; 郑玉莹; 关敏怡; 彭维; 苏薇薇

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补肺活血胶囊中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的测定方法

论文 | 更新时间：2023-12-11

- 补肺活血胶囊中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的测定方法
- Determination of Calycosin-7-O-β-D-glucoside and Calycosin in Bufeihuoxue Capsules by HPLC
- 中山大学学报(自然科学版)(中英文) 2017年56卷第6期页码：123-127
- 作者机构：
  
  中山大学生命科学学院,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2017-11-25，
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唐清, 朱晓枭, 郑玉莹, 等. 补肺活血胶囊中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的测定方法[J]. 中山大学学报(自然科学版)(中英文), 2017,56(6):123-127.

TANG Qing, ZHU Xiaoxiao, ZHENG Yuying, et al. Determination of Calycosin-7-O-β-D-glucoside and Calycosin in Bufeihuoxue Capsules by HPLC[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2017,56(6):123-127.
唐清, 朱晓枭, 郑玉莹, 等. 补肺活血胶囊中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的测定方法[J]. 中山大学学报(自然科学版)(中英文), 2017,56(6):123-127. DOI：

TANG Qing, ZHU Xiaoxiao, ZHENG Yuying, et al. Determination of Calycosin-7-O-β-D-glucoside and Calycosin in Bufeihuoxue Capsules by HPLC[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 2017,56(6):123-127. DOI：

摘要

采用HPLC构建测定补肺活血胶囊中毛蕊异黄酮葡萄糖苷和毛蕊异黄酮的方法。以Hitachi High-Tech C

（5 μm，4.6 mm×250 mm）为色谱柱，以乙腈-φ=02%甲酸溶液为流动相，梯度洗脱，检测波长为260 nm，色谱柱温度为25 ℃，流速为0.8 mL/min。经方法学验证，毛蕊异黄酮葡萄糖苷、毛蕊异黄酮分别在0.022 5~0.901 8 μg (R

=1)、0.007 5~0.299 0 μg (R

=1) 范围内线性关系良好，二者的平均回收率分别为93.97% (RSD=3.46%)、90.04% (RSD=3.25%)。结果表明，所建立的方法简单、快捷，适用于补肺活血胶囊中毛蕊异黄酮葡萄糖苷和毛蕊异黄酮的测定。

Abstract

The HPLC method was established for determination of Calycosin-7-O-β-D-glucoside and Calycosin in Bufeihuoxue Capsules. The separation was performed on Hitachi High-Tech C

(5 μm，4.6 mm×250 mm) column eluted with the mobile phase consisting of acetonitrile and 02% formic acid solution in gradient elution mode at the flow rate of 0.8 mL/min. The detection wavelength was 260 nm and the column temperature was 25 ℃. The method was validated and shown that Calycosin-7-O-β-D-glucoside and Calycosin had a good linear relationship in the range of 0.022 5~0.901 8 μg (R

=1)

0.007 5~0.299 0 μg(R

=1)

respectively. The average recovery rates were 93.87% (RSD=3.46%)、90.04% ( RSD=3.25%)

respectively. The established method is simple

rapid and reproducible

which is suitable for the determination of Calycosin-7-O-β-D-glucoside and Calycosin from Bufeihuoxue Capsules.

关键词

高效液相色谱补肺活血胶囊毛蕊异黄酮葡萄糖苷毛蕊异黄酮测定

Keywords

high performance liquid chromatographyBufeihuoxue CapsuleCalycosin-7-O-β-D-glucosideCalycosindetermination

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