Conotoxin lt1c was identified from the cDNA library of venom gland of  Conus litteratus .To obtain enough lt1c and investigate the bioactivities

conotoxin lt1c was recombinant and expressed in  Escherichia coli  by fusion with TRX. Conotoxin lt1c was purified by affinity chromatography

enzyme digestion and gel filtration chromatography. The recombinant lt1c showed obvious inhibition activity on the neuromuscular transmission on the frog sciatic/cutaneus pectoris preparation at the concentration of 6 μmol/L in 30 min. lt1c appeared potent and longer-lasting analgesics in the hotplate pain model mice. Additionally

lt1c showed toxicity to mice by intraventricular injection. The successful expression of lt1c with potential analgesicactivity provided the possibility for further pharmaceutical research.