Culturable bacteria in the sediment of Huixian wetland were isolated by dilution plate coating method and analyzed with 16S rRNA gene sequences. The results showed that total 65 isolates

subordinate to 4 phylums

12 genera

24 species

were obtained. These strains belonged to five genera (Bacillus

Paenibacillus

Fictibacillus

Lysinibacillus and Enterococcus) in Firmicutes

two genera (Streptomyces and Rhodococcus) in Actinobacteria

four genera (Pseudomonas

Stenotrophomonas

Pantoea and Delftia) in Proteobacteria and one genus (Chryseobacterium) in Bacteroidetes. The predominant microflora is Bacillus sp. strains

occupying 53.85% of the all the isolates

which conforms to the fact that Huixian wetlands are becoming eutrophic. Totally four kinds of conditional pathogenic bacteria are detected

accounting 15.38% of the isolate

indicating that the ecological environment has been negatively affected by the human activity. There is  another one bacteria strain tolerant to PCNB in 300 mg/L or more

and 30 isolates from 12 Genus have arsenic tolerance. This appearance might have direct relationship with local pesticide and fertilizer abuse.