摘要:The brown planthopper (BPH, Nilaparvata lugens) is an important rice pest with high fecundity, strong adaptability and migratory ability. In order to obtain safe and efficient RNAi target genes, we established a set of process for RNAi target gene screening, and 32 candidate target genes were obtained,of which 12 were tested for RNAi efficiency. Furthermore,we investigated the control effects of a endosomal sorting complex which was required for transport III subunit Snf7 (NlSnf7). NlSnf7 gene was expressed in various stages and tissues of BPH. After RNA interference of NlSnf7, its mRNA expression level was significantly reduced, and the corrected mortality rate of BPH reached 70% on the 13th day after injection of dsNlSnf7 at 50 ng/head, and 48% on the 9th day after feeding of dsNlSnf7 at 50 ng/μL. NlSnf7 protein was also significantly reduced on the 5th day after injection of dsNlSnf7. In addition, when dsNlSnf7 was injected into Ostrinia furnacalis and Sogatella furcifera in which no 21 bp continuously identical sequence was found, there were no significant changes between the experimental group and the control group. These results indicated that dsNlSnf7 fragment is an efficient and safe RNAi target molecule.
关键词:Nilaparvata lugens;RNAi target genes;endosomal sorting complex required for transport Ⅲ subunit Snf7
摘要:Acute myeloid leukemia (AML) is a kind of aggressive hematopoietic malignancies. Understanding the molecular mechanism of AML generation and progression is necessary to develop novel therapies for the disease. LAMP4 is a member of the lysosomal related membrane protein family, and its diverse roles in tumor progression have been reported different in certain cancers. In this study, we found that LAMP4 expressed a high level in AML patient samples compared with those controls, suggesting its potential as a biomarker for AML. Functional studies showed that knocking down LAMP4 can significantly promote the cell apoptosis and differentiation of AML cells. Furthermore, LAMP4 was found to locate at lysosome, and it can be dropped from the lysosome under the Phorbol 12-myristate 13-acetate (PMA) treatment, indicating that the function of LAMP4 suppressing the cell differentiation was related to its lysosomal localization. The study suggested that LAMP4 could be served as a potential biomarker and target for AML.
摘要:Phytochemical investigation on the pericarps of Garcinia mangostana resulted in the isolation of 22 xanthones. On the basis of the spectroscopic data, the structures of these known compounds were identified as 1,3,7-trihydroxyxanthone (1), 1,3,6,7-tetrahydroxy-8-prenylxanthone (2), 1,3,5-trihydroxy-4-prenylxanthone (3), 8-deoxygartanin (4), cudraxanthone G (5), gartanin (6), 6-deoxy-γ-mangostin (7), γ-mangostin (8), α-mangostin (9), 1,3-dihydroxy-6,7-dimethoxy-2,8-diprenylxanthone (10),β-mangostin (11), garcinone D (12), garcinone B (13), mangostenone D (14), 3-O-methylmangostenone D (15), 9-hydroxycalabaxanthone (16), 11-hydroxy-1-isomangostin (17), brasilixanthone B (18), garcimangosxanthone D (19), BR-xanthone A (20), tovophyllin A (21), and 1,3,6-trihydroxy-2,5-bis(3-methylbut-2-enyl)-6',6'-dimethyl-4',5'-dihydropyrano[2,3':7,8]xanthone (22). Compounds 2-22 were prenylated xanthones, and 3 and 15 were isolated from this plant for the first time. The neuroprotective effects of all compounds against glutamate-induced cell death were tested in murine hippocampal neuronal cell line HT22.
摘要:As a new technology of sludge dehydration, electro-osmotic dehydration has the advantages of good dehydration effect, low cost and without flocculant. Through two rounds of orthogonal experiments and by using moisture content of sludge and dewatering energy consumption as evaluation system, this study investigated the combination of optimal operating parameters of mechanical pressure, electroosmosis time, voltage gradient and sludge thickness. After shrinking range of parameters by the first orthogonal experiment, the second orthogonal experiment was carried out. Finally, the optimal operation parameters were determined as follows: mechanical pressure was between 14.6 and 15.0 kPa, and electroosmosis time was between 58.7 and 70 min, and voltage gradient was between 15.1 and 16.1 V/cm, and sludge thickness was 0.50 cm. After dewatering, the moisture content of sludge was between 55.00% and 58.00%, and ewatering energy consumption was between 0.110 and 0.120 kW·h/kg. The optimal operation parameters obtained through two rounds of orthogonal experiments, the moisture content of sludge can achieve the standards and energy consumption is only 9% to 19% of thermal drying.
关键词:municipal sludge;electro-osmosis dewatering;two rounds of orthogonal test;parameter
摘要:Phthalate esters (PAEs) are a kind of important global environmental pollutants. It is particularly important to find a broad spectrum of enzymes that degrade PAEs efficiently and have good environmental adaptability. A novel esterase gene est924 was cloned from microorganisms in soil by metagenomic technique, with the highest homology of 62.42% to the reported alpha/beta-hydrolase derived from Synechococcus sp.cc9311. The gene encoding est924 was obtained by PCR amplification, the recombinant plasmid was obtained by ligating the plasmid pET-41a(+) with est924. Then, the recombinant plasmid was transformed into E. coli BL21 (DE3) for heterologous expression, and the recombinant esterase Est924 was obtained. The enzymatic properties of Est924 and its efficiency to degrade PAEs were studied. The results showed that Est924 obtained in this study was a new alkali-resistant esterase and had good degradation activities to dimethyl phthalate (DMP), diethyl phthalate (DEP), dipropyl phthalate (DPrP), dibutyl phthalate (DBP), diamyl phthalate (DPP) and dihexyl phthalate (DnHP). Therefore, Est924 has a great potential for PAEs bioremediation.
摘要:The environment features under unstable conditions, water vapor, energy and dynamics conditions were studied by using sounding data and conventional observation data for 54 warm-sector heavy rainfall cases in South China from April to June during 2008-2017. The thresholds of key environmental parameters to distinguish the significantly strong warm-sector heavy rainfall cases are extracted with percentile method. The conclusions are shown as follows: (1) The depression of dew point at 850 hPa, the atmospheric precipitation and the deep vertical wind shear can be used to predict the occurrence of warm-sector heavy rainfall in South China. The water vapor in the lower troposphere is nearly saturated with high atmospheric precipitation before the occurrence of warm-sector heavy rainfall. Combined with certain lifting conditions, the latent heat can be released from water vapor condensation in the atmosphere, so that the convection can continue to form rainstorm. The thresholds of physical parameters include depression of dew point≤2.2 ℃ at 850 hPa, atmospheric precipitation≥56 mm and deep vertical wind shear ≥9 m/s. The above conditions are favorable for the generation of warm-sector heavy rainfall. (2) Compared with common warm-sector heavy rainfall, the significantly strong warm-sector heavy rainfall is of more sufficient water vapor in the lower troposphere. Under the condition of depression of dew point≤2.0 ℃ at 850 hPa, the probability of significantly strong warm-sector heavy rainfall is higher.
关键词:warm-sector heavy rainfall in South China;sounding data;physical parameter;environment conditions
摘要:In order to investigate the creep characteristics of colluvial soil-rock mixture, shear creep tests were performed by using a large-scale direct-shear instrument on a colluvial soil-rock-soil mixture of a slope in Chenggu county, Shaanxi province. The creep characteristics of the colluvial soil-soil-mixture under graded loading were mainly studied to establish the Burgers creep model and determine the parameters through the data fitting. The method of obtaining the final creep value of separate loading under the condition of graded loading is suggested. The results showed that the creep law of soil-rock mixture exhibits a gradual creep behavior under the grading loading, and the creep deformation increases with decreasing vertical and increasing shear stresses. The curves of -t under different vertical stresses are all highly linear, and there is no obvious correlation between the slope of the fitted straight line and the shear stress. The Burgers model proposed in this paper can well simulate the shear creep characteristics of the colluvial soil-rock mixture in the research area.
关键词:colluvial soil-rock mixture;shear creep;Burgers model
摘要:The study used the measured precipitation of 74 meteorological stations in the Pearl River Basin to validate the accuracy of TRMM (Tropical Rainfall Measuring Mission) precipitation data on annual, monthly, and daily scales, and analyzed the temporal and spatial distribution characteristics of TRMM monthly precipitation. The results show that the correlation coefficients of TRMM precipitation data in the Pearl River Basin are 0.918 and 0.940, and the average relative errors are 10.87% and 22.01% with higher accuracy on annual and monthly scales overall, while the correlation coefficient of daily precipitation data is 0.457 with poor accuracy and the average relative error is 113.62%. For the TRMM monthly precipitation data from a single station, most of the data have higher correlation coefficient and less error with correlation coefficient above 0.9 and relative error below 15%, and the average relative error of each sub-basin is less than 13% but the data errors from Yuanjiang, Nanning, Baise, Luoding and Longzhou stations are relatively large. The spatial distribution characteristics and trends of annual average precipitation in the Pearl River Basin from TRMM and meteorological stations are consistent, and the differences are mainly distributed in the upstream with complex terrain and coastal areas. In the spatial distribution of TRMM precipitation, the average annual precipitation in the Pearl River Basin generally increases from the northwest area to the southeast area, and the difference in precipitation between different regions is extremely obvious. In terms of temporal distribution, the precipitation is higher from April to October, and less from November to February.
关键词:TRMM;precipitation;the Pearl River basin;accuracy validation;temporal and spatial distribution
摘要:Based on the daily temperature data of 47 meteorological stations in the Qinling-Daba mountains and adjacent areas from 1960 to 2017, the spatial-temporal characteristics of extreme temperature were analyzed by using climate tendency rate, correlation analysis and M-K mutation test. The results are as follows : (1) In the past 58 years, the extreme temperature extreme index, summer days, hot night days, warm day (night) days, heat duration days, and biological growth season days tended to increase, the extreme low temperature index tended to decrease. (2) The spatial variation of extreme temperature changes in the northern and southern Qinling Mountains is significant; the extreme temperature index in the northern and eastern Qinling Mountains increased in response to climate change more obviously than in the southern and western Qinling Mountains. The number of freezing days, frost days and cold days in the northern and adjacent areas showed a downward trend; the number of summer days, warm days (nights) and heat-sustaining days in the eastern and northern Qinling Mountains showed an upward trend. (3) The average temperature changed suddenly in 1996. The effects of extreme temperature events on the northern and southern Qinling Mountains were different. The extreme high temperature index showed a significant increase trend, and the extreme low temperature index showed a decreasing trend, indicating that there is a good correlation between extreme temperature changes and climate warming. (4) Pearson correlation indicated that all the extreme temperature indices had a good correlation, except for the extreme indices. (5) The Hurst index results show the same direction change trend of extreme temperature index in the northern and southern Qinling Mountains.
关键词:extreme temperature index;trend change;spatial difference;the northern and southern Qinling mountains
摘要:In order to investigate the variation of light-induced-degradation (LID) and regeneration of industrial Passivated Emitter and Rear Cells (PERC solar cells) with the positions of silicon wafers on a silicon rod, six groups of silicon wafers were cut from one industrial boron-doped Cz-Si rod from top to bottom with a certain distance. After measuring concentration of boron, oxygen, carbon and transition metal impurities as well as minority carrier lifetime, the wafers were made into PERC solar cells using standard industrial processes. Then, the changes of their parameters with time were measured by using a solar cell I-V characteristic tester during the 1st LID (45 ℃, 1 sun, 12 h), regeneration (100 ℃, 1 sun, 24 h) and 2nd LID (45 ℃, 1 sun, 12 h). The results show that the PERC solar cells made from bottom silicon wafers show the largest rising extent in efficiency, open-circuit voltage and short-circuit current during the regeneration, and possess the highest efficiencies with only slight LID at the initial stage during the 2nd LID. The results demonstrate that the regeneration treatment with the condition of 100 ℃, 1sun light intensity, and 24 hours can nearly completely inactivate B-O defects inside the PERC solar cells. The slight LID of the efficiencies at the initial stage of the 2nd LID process can be attributed to the boon-oxygen defects not reaching regeneration state yet, which confirm that boron oxygen defects at the regeneration state have good anti-LID performance at the meantime.
关键词:boron-doped Czochralski silicon;PERC solar cell;light induced degradation (LID);regeneration
摘要:Transmission switching is one of the effective methods to control transmission congestion by using the existing infrastructure. In order to ensure the security of system node voltage while breaking some lines, a co-optimization model of unit commitment and transmission switching considering AC power flow constraints is proposed in the transmission network with high proportion of renewable energy. On the premise of ensuring security of the transmission network, it can improve the efficiency of the consumption proportion of renewable energy and reduce of system operation cost. In this paper, the transmission network generation cost and the minimum penalty for wind and photovoltaic power are taken as the objective functions, and the chance constraints are used to deal with the uncertainty of wind power and photovoltaic power. A simulation example is established for IEEE-RTS 24 node system. Based on the typical daily load, wind power and photovoltaic predicted power data, the generalized benders decomposition method is used to solve the model. The results show that this model can effectively improve the consumption ratio of renewable energy, reduce the operating cost of the system, and ensure the safety of the node voltage, which can be used to guide the unit commitment with high safety requirements, and has a certain engineering practical value.
关键词:ac power flow;unit commitment;transmission switching;generalized benders decomposition
摘要:It is proposed and verified that the information entropy is a quantitative standard for lexical richness. Firstly, the English novels are categorized into four groups, namely, magic/science fiction, mystery novels, humorous satirical novels, and children's literature. Then the authors calculate the information entropy of each English novel, compare the information entropy of the four groups by means of graphs, and observe whether the difference of information entropy among these four categories consists with what the authors' expectation. Through verification, the authors find that the information entropy of children's literature is averagely the lowest, and the information entropy of magic/science fiction is generally higher. According to previous studies and our usual reading experience, the magic/science fiction indeed has higher vocabulary richness, and the vocabulary richness in children's literature is lower. Finally, the authors use hypothesis testing to verify the difference of entropy among the categories. Then, the authors conclude that information entropy can be used as an indicator of the vocabulary richness.
摘要:Great success in deep-learning-based style transfer is accelerating the development of photo artistic stylization applications. And the change of loss function from per-pixel loss to perceptual loss based on the Gram matrix is the most critical part of this progress. Gram matrix shows good performance in style feature extraction, but it only focuses on correlations among same level features. Therefore, Gram matrix cannot be considered as a complete representation of styles. However, most of the research focus on how to improve transfer speed by designing new model structure instead of analyzing and modifying the Gram matrix. The cross-layer correlation matrix is used to calculate style loss function as a replacement or supplement to the Gram matrix. By experiments, it is shown that this method can reduce 20% of the calculation time in comparison with the Gram matrix method while yielding similar outputs.
关键词:style transfer;Gram matrix;convolutional neural network;style loss function;perceptual loss;deep learning
摘要:It’s very difficult to establish the global existence in the framework of due to the interaction between collision operators and the underlying singularity at the boundary. In this paper, we construct a global and unique solution in a new function space for the non-cutoff Boltzmann equation with inflow boundary condition in a finite channel. Moreover, we also obtain the large-time behavior and the propagation of the regularity of the solution.
摘要:Vibration frequency describes the structure's vibration characteristics. Since frequency data can be easily obtained, it's often applied in the identification of structural damage. However, there's a defect that the amount of frequency data is usually small, which would cause the identification to be ill-posed. This paper proposes a new method in damage identification using frequency data. In this method, we introduce sparse regularization to overcome the ill-posedness of the problem. The proposed damage identification approach can work for merely low order frequency data. Damage identification of a cantilever beam is conducted to verify the proposed approach. As a result, the exact position of the damage can be determined, which proves the approach to be functional and accurate.
关键词:damage identification;sparse regularization;vibration frequency data;alternating minimization approach;threshold setting method
摘要:By using the characteristic line method, the weak well-posedness of the Cauchy problem of the two-component b family system is obtained when the initial value . Firstly, the b family system is transformed into an ODE system. Then, the existence and uniqueness of the solution of the ODE system are obtained by the existence and uniqueness theory of the solution. Finally, the existence and uniqueness of the solution of the b family system are obtained by the relationship between the two-component b family system and the ODE system. Moreover, the stability of the solutions with respect to the initial value is given.
关键词:two-component b family system;weak well-posedness;characteristic line method
摘要:A class of fourth-order two-point boundary value problems with parameters are studied, where the parameters and ,,.Under certain conditions of the nonlinear term , the existence of at least three non-negative solutions for the discussed problem are obtained by using fixed point results of Leggett-Williams type.
关键词:fourth-order;multiple positive solutions;Leggett-Williams fixed point theorem