The study aimed to determine single nucleotide polymorphism of 21Y-chromosomal loci in seven ethnic minorities

including Shui

Bouyei

Dong

Gelo

Zhuang

Maonan and Mulam

of Zhuang-Dong language in the Guizhou province of China

and explore differences in the polymorphism from other Language ethnics. The SNapShot method was used to detect the complex amplicons of the 21 Y-SNPs loci in 445 males from seven Guizhou Zhuang-Dong language ethnics. Frequencies of alleles

haplotypes

and haploid populations were calculated using a direct counting method. SPSS 24 was used for principal component analyses. The haplotype diversity values were 0.597 7

0.945 6

0.885 6

0.886 9

0.768 6

0.358 7 and 0.707 8

respectively

in the seven ethnics. We performed principal component analyses based on the frequencies of haplogroups by comparing our data with those reported for other nine ethnic groups in China. The results showed that Zhuang-Dong Language and Hmong-Mien Language were clustered together. From the perspective of patrilineal genetics

the relationship of ZhuangDong Language to Hmong-Mien Language is relatively closer than those of other ethnics.