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The regulatory effects of exogenous boron on the cadmium absorption and antioxidant enzyme and AsA-GSH cycle key enzyme activity in Chinese flowering cabbage
增强出版- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 63, Issue 6, Pages: 141-149(2024)
- 作者机构：
1.广东省农业科学院农业质量标准与监测技术研究所，广东 广州 510640
2.农业农村部农产品质量安全风险评估实验室（广州），广东 广州 510640
3.岭南现代农业科学与技术广东省实验室河源分中心，广东 河源 517000
4.广东农科监测科技有限公司，广东 广州 510640
- 作者简介：
- 基金信息：
DOI：10.13471/j.cnki.acta.snus.ZR20240166
CLC： Q89Published：25 November 2024，
Published Online：23 July 2024，
Received：20 May 2024，
Accepted：27 May 2024
- 稿件说明：
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李富荣,王琳清,曹怡然等.硼对菜心镉吸收及抗氧化酶和AsA-GSH循环关键酶活性的调控效应[J].中山大学学报(自然科学版)(中英文),2024,63(06):141-149.
LI Furong,WANG Linqing,CAO Yiran,et al.The regulatory effects of exogenous boron on the cadmium absorption and antioxidant enzyme and AsA-GSH cycle key enzyme activity in Chinese flowering cabbage[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2024,63(06):141-149.
李富荣,王琳清,曹怡然等.硼对菜心镉吸收及抗氧化酶和AsA-GSH循环关键酶活性的调控效应[J].中山大学学报(自然科学版)(中英文),2024,63(06):141-149. DOI： 10.13471/j.cnki.acta.snus.ZR20240166.
LI Furong,WANG Linqing,CAO Yiran,et al.The regulatory effects of exogenous boron on the cadmium absorption and antioxidant enzyme and AsA-GSH cycle key enzyme activity in Chinese flowering cabbage[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2024,63(06):141-149. DOI： 10.13471/j.cnki.acta.snus.ZR20240166.
摘要
针对华南地区旱地土壤低硼高镉和蔬菜重金属镉污染日益严重的现状，以广东特色蔬菜菜心（
Brassica parachinensis
L）为研究对象，通过水培实验研究了外源硼添加对菜心生长特性和镉吸收的调控效应，并从抗氧化酶和AsA-GSH循环关键酶活性的角度探讨硼调控蔬菜镉胁迫的生理机制。研究结果显示，在5 μmol/L的镉处理下，20和50 μmol/L的外源硼添加显著提高菜心株高和根长达50%以上，同时，50 μmol/L的外源硼添加较0.25 μmol/L硼添加时的地上部和地下部生物量都明显增加114.19%和187.59%，说明适当施加硼元素供应能有效缓解重金属镉对蔬菜的胁迫作用。另外，10、20、50 μmol/L 3种浓度的外源硼添加都不同程度地减少了菜心地上部和地下部的镉吸收，下降量达24.0%。从硼影响菜心的抗氧化酶活性和AsA-GSH循环关键酶活性的结果来看，镉胁迫下添加不同浓度外源硼处理后，不同菜心抗氧化酶和AsA-GSH循环关键酶活性变化规律存在一定差异。本文研究结论为，适宜浓度的硼添加可有效提高镉胁迫下的菜心地上部分和地下部分的SOD、POD、CAT、APX、DHAR、GR活性，增强菜心的抗氧化能力，促进其AsA-GSH循环，从而降低镉对菜心植株的胁迫作用并调控菜心对镉的吸收。这意味着有可能通过筛选适合的硼肥类别和施用量，进一步实现控制蔬菜可食部分镉含量在安全限量范围内，研究结论可为合理施用硼肥保障中轻度镉污染土壤上的蔬菜安全种植提供科学的参考依据。
Abstract
To solve the present situation of low boron and high cadmium in dryland soils and increasingly serious cadmium pollution in vegetables in southern China， in this study， we chose the Guangdong specialty vegetable Chinese flowering cabbage （
Brassica parachinensis
L） as the research object and performed to study the regulatory effects of exogenous boron addition on its growth characteristics and cadmium absorption. The vegetable physiological mechanism of boron regulation under cadmium stress was also explored from the perspectives of antioxidant enzymes and AsA-GSH cycle key enzyme activities. The results showed that under 5 μmol/L cadmium treatment， 20 and 50 μmol/L exogenous boron addition significantly increased the plant height and root length of Chinese flowering cabbage by more than 50%， and the aboveground and underground biomass was increased significantly by 114.19% and 187.59% with 50 μmol/L boron addition compared with the 0.25 μmol/L boron. The results indicated that appropriate application of boron supply would effectively alleviate the cadmium stress on vegetable growth. Additionally， the addition of exogenous boron as 10， 20 and 50 μmol/L could reduce cadmium absorption to 24.0% in the aboveground and underground parts of Chinese f
lowering cabbage. We also found that there were differences in the activity changes of antioxidant enzymes and AsA-GSH cycle key enzymes with different exogenous boron addition under cadmium stress. Overall， it can be concluded that the appropriate concentration of boron addition could effectively improve the SOD， POD， CAT， APX， DHAR and GR activities of the aboveground and underground parts of Chinese flowering cabbage under cadmium stress， to enhance its antioxidant capacity and promote its AsA-GSH cycle， thereby reducing the stress effect and cadmium absorption. Accordingly， screening suitable boron fertilizer categories and application rates would further achieve control over the cadmium contents in edible parts of vegetables within their safe limits. This conclusion would provide a scientific reference for rational application of boron fertilizer to ensure the safe planting of vegetables on farmland soil with moderate to mild cadmium pollution.
关键词
硼菜心（Brassica parachinensis L）镉酶活性AsA-GSH循环
Keywords
boronChinese flowering cabbage （Brassica parachinensis L）cadmiumenzymatic activityAsA-GSH cycle
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