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NO production improvement and anti-diabetic effects of YouthyCome composite herbal tea
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 62, Issue 4, Pages: 65-72(2023)
- 作者机构：
1.暨南大学药学院，广东 广州 510632
2.广州药本君安生物技术有限公司，广东 广州 510663
- 作者简介：
- 基金信息：
DOI：10.13471/j.cnki.acta.snus.2022E030
CLC： R961Published：25 July 2023，
Published Online：10 April 2023，
Received：19 September 2022，
Accepted：24 December 2022
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陈世坚,刘志军,陈河如.美亚通复合植物茶促NO生成及其降糖作用[J].中山大学学报(自然科学版),2023,62(04):65-72.
CHEN Shijian,LIU Zhijun,CHEN Heru.NO production improvement and anti-diabetic effects of YouthyCome composite herbal tea[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(04):65-72.
陈世坚,刘志军,陈河如.美亚通复合植物茶促NO生成及其降糖作用[J].中山大学学报(自然科学版),2023,62(04):65-72. DOI： 10.13471/j.cnki.acta.snus.2022E030.
CHEN Shijian,LIU Zhijun,CHEN Heru.NO production improvement and anti-diabetic effects of YouthyCome composite herbal tea[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(04):65-72. DOI： 10.13471/j.cnki.acta.snus.2022E030.
摘要
为探讨美亚通复合植物茶（YCHT）的促NO生成及降糖作用。选取H9C2、PC-12细胞、HUVEC细胞，加入不同质量浓度的YCHT孵育24 h后，检测各质量浓度YCHT对3种细胞的存活率以及细胞内外NO的含量；在正常大鼠体内给予YCHT后，观察其对正常动物空腹血糖的影响；以链脲佐菌素诱导SD大鼠建立糖尿病大鼠模型，分析各组动物的空腹血糖值；通过建立高糖果蝇模型，以体质量、海藻糖为检测指标，观察YCHT对高糖果蝇的影响。结果显示，YCHT作用于H9C2、PC-12、HUVEC细胞24 h后存活率为80%以上的质量浓度分别为3.12、1.56和3.12 mg/mL；YCHT在3.5 mg/mL的质量浓度下分别作用于H9C2和HUVEC细胞24 h，或1.5 mg/mL的质量浓度下作用于PC-12细胞，细胞上清液与细胞裂解液中的NO含量均显著升高，差异具有统计学意义（
P<
0.05）；在每kg体质量5.1 g剂量下，正常SD大鼠的初始体质量、初始空腹血糖值、实验终期的体质量、空腹血糖值与空白对照组相比，无显著差异（
P>
0.05）；在低剂量受试组（每kg体质量0.85 g剂量）中，实验终期的空腹血糖值显著降低（
P
<
0.05）；同时，
m
（YCHT）꞉
m
（培养基）=5%受试组还降低了高糖果蝇体质量及海藻糖水平（
P
<
0.05）。结果表明，YCHT在适当质量浓度下不影响H9C2、PC-12、HUVEC细胞的存活率，并能促进H9C2、PC-12、HUVEC细胞增加NO的生成；每kg体质量5.1 g剂量的YCHT对正常SD大鼠的体质量及空腹血糖值没有影响；合适剂量的YCHT能逆转高糖SD大鼠空腹血糖值的升高，以及逆转高糖果蝇体质量和海藻糖水平的增加。
Abstract
To investigate both NO production improvement and anti-diabetic effects of YouthyCome composite herbal tea （YCHT）， in this study， H9C2 cells， PC-12 cells and HUVEC cells were selected and incubated with different concentrations of YCHT for 24 hours， respectively. Cell survival and the content of nitric oxide （NO） inside and outside the cells were detected. After administration of YCHT to normal rats， the effect on fasting blood glucose was observed. The diabetic SD rats was set up by inducing with streptozotocin. The blood glucose value and the percentage of blood glucose decrease in each group were analyzed. By establishing high glucose drosophila model， the anti-diabetic effect of YCHT was further evaluated using body mass and trehalose level as indicators. The results showed that after treated with YCHT at a mass concentration up to 3.12，1.56 and 3.12 mg/mL corresponding to H9C2， PC-12， and HUVEC cells for 24 hours， respectively， their survival rates still maintained above 80%. It was found that after H9C2 and HUVEC cells were treated with 3.5 mg/mL YCHT， respectively for 24 hours， as well as PC-12 cells were treated with 1.5 mg/mL YCHT for 24 hours， the contents of NO in both cell supernatant and cell lysate increased significantly （
P<
0.05
）
. Compared the indicators including initial body mass， initial fasting blood glucose level， final experimental body mass and fasting blood glucose level， there was no significant difference between normal SD rat treated with 5.1 g/kg BW YCHT group and blank control group （
P＞
0.05）
.
In the low dose group （0.85 g/kg BW）， the value of fasting blood glucose decreased significantly at the end of the experiment （
P<
0.05）
.
In the meantime， treatment of YCHT amount to 5% culture mass decreased the body mass and trehalose content of high glucose drosophila melanogaster （
P<
0.05）. The results indicate that appropriate dosage of YCHT does not affect the survival of H9C2， PC-12， HUVEC cells， but improves the NO production in the cells. Treatment of YCHT at the dose of 5.1 g/kg BW to normal SD rats does not affect their body mass and fasting blood glucose. However， treatment of appropriate dose of YCHT reverses the increase of fasting blood glucose in diabetic SD rats， and the increases of body mass and trehalose content in diabetic drosophila.
关键词
美亚通复合植物茶药食同源糖尿病降糖作用NO糖尿病预防
Keywords
YouthyCome composite herbal teahomology of medicine and fooddiabetes mellitusanti-diabeticsNOdiabetics prevention
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