The <italic style="font-style: italic">ttk</italic> gene structural characteristics of <italic style="font-style: italic">Sogatella furcifera</italic> and its expression level in nymphs

YANG Mei; FENG Bo; WANG Fanghai

doi:10.13471/j.cnki.acta.snus.2022E019

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The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs

Research articles | 更新时间：2023-11-01

- The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 62, Issue 2, Pages: 47-50(2023)
- 作者机构：
  
  有害生物控制与资源利用国家重点实验室 / 中山大学生命科学学院，广东广州 510275
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2022E019
  CLC： Q965
- Published：25 March 2023，
  
  Published Online：08 November 2022，
  
  Received：08 July 2022，
  
  Accepted：05 August 2022
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杨梅,冯博,王方海.白背飞虱ttk结构特点及在若虫中的表达[J].中山大学学报(自然科学版),2023,62(02):47-50.

YANG Mei,FENG Bo,WANG Fanghai.The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(02):47-50.
杨梅,冯博,王方海.白背飞虱ttk结构特点及在若虫中的表达[J].中山大学学报(自然科学版),2023,62(02):47-50. DOI： 10.13471/j.cnki.acta.snus.2022E019.

YANG Mei,FENG Bo,WANG Fanghai.The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2023,62(02):47-50. DOI： 10.13471/j.cnki.acta.snus.2022E019.

摘要

ttk

基因转录产物为重要的转录因子，可以调控生物体的发育、分化、性行为等。本研究从自建的转录组数据库中比对到一条来源于白背飞虱（

Sogatella furcifera

）

ttk

的cDNA序列，全长为1 657 bp，其开放阅读框序列为1 377 bp，编码由458个氨基酸组成的蛋白质，具有典型的BTB结构域和锌指结构。系统进化树显示白背飞虱

ttk

与六星灯蛾（

Zygaena filipendulae

）、温带臭虫（

Cimex lectularius

）和褐飞虱（

Nilaparvata lugens

）的同源性较近。定量检测结果显示，

ttk

在1龄若虫期含量较高，2龄则下降到较低的水平，从3龄开始，随着龄期的增长，表达量则逐渐增加，到5龄若虫时，

ttk

表达量是3龄若虫的2倍多。这是第一次详细报道白背飞虱

ttk

基因的结构和表达水平，为今后进一步研究该基因的具体生物学功能提供了基础。

Abstract

Tramtrack（

ttk

） gene transcription products are important transcription factors， which can regulate the development， differentiation and sexual behavior of organisms. In this study， a 1 657 bp cDNA sequence of

ttk

was identified from the transcriptome database of

Sogatella furcifera

. The open reading frame is 1 377 bp in length and encodes a protein composed of 458 amino acids. It has a typical BTB domain and a zinc finger structure. The phylogenetic tree shows that

ttk

S. furcifera

is closely homologous to that of

Zygaena filipendulae

，

Cimex lectularius

and

Nilaparvata lugens

. Quantitative detection results showed that the transcriptional level of

ttk

was higher in the 1st instar nymphs， but decreased to a lower level in the 2nd instar nymphs. From the 3rd instar，

ttk

expression increased gradually with instar growth， and at the 5th instar nymphs，

ttk

expression was more than twice as high as that in the 3rd instar nymphs. This is the first in detail investigation on

ttk

gene structure of white backed planthopper， and the results provide a foundation for further and function and regulation study of

ttk

gene， in the growth and development of rice planthopper in the future.

关键词

tramtrack（ttk）白背飞虱（Sogatella furcifera）若虫基因表达

Keywords

tramtrack（ttk）Sogatella furciferanymphgene expression

references

唐楠， 2017. 白背飞虱基因组测序及其受南方水稻黑条矮缩病毒侵染的转录组分析［D］. 合肥：中国科学技术大学.

杨梅，何静怡，林佳，等， 2020. 昆虫母体效应基因功能研究进展［J］. 应用昆虫学报， 57（3）： 568-573.

ALBAGLI O， DHORDAIN P， DEWEINDT C， et al， 1995. The BTB/POZ domain： A new protein-protein interaction motif common to DNA and act in binding proteins ［J］. Cell Growth and Differ， 6： 1193-1198.

ALI E， LIAO X， YANG P， et al， 2017. Sublethal effects of buprofezin on development and reproduction in the white-backed planthopper， Sogatella furcifera （Hemiptera：Delphacidae）［J］. Sci Rep， 7（1）： 16913.

BONCHUK A， DENISOV S， GEORGIEV P， et al，2011. Drosophila BTB/POZ domains of “ttk group” can form multimers and selectively interact with each other［J］. J Mol Biol， 412（3）： 423-436.

CHAHARBAKHSHI E， JEMC J C， 2016. Broad-complex， tramtrack， and bric-à-brac （BTB） proteins： Critical regulators of development［J］. Genesis， 54（10）： 505-518.

CHENG D J， QIAN W L， MENG M， et al， 2014. Identification and expression profiling of the BTB domain-containing protein gene family in the silkworm， Bombyx mori［J］. Int J Genomics， 2014：865065.

HARRISON S D， TRAVERS A A，1990. The tramtrack gene encodes a Drosophila finger protein that interacts with the ftz transcriptional regulatory region and shows a novel embryonic expression pattern［J］. EMBO J， 9（1）： 207-216.

HUANG J F， FANG L， WANG S， et al， 2019. Molecular cloning， expression profiling， and functional analysis of a broad-complex isoform 2/3 （Br-Z2/Z3） transcription factor in the diamondback moth， Plutella xylostella （L.）［J］. Arch Insect Biochem and Physiol， 101（2）： e21549.

ISHII K， WOHL M， DESOUZA A， et al. 2020. Sex-determining genes distinctly regulate courtship capability and target preference via sexually dimorphic neurons［J］. eLife， 9： e52701.

KNAPP E M， LI W， SUN J J， 2019. Downregulation of homeodomain protein cut is essential for Drosophila follicle maturation and ovulation［J］. Development， 146（18）： dev179002.

SATO K， GOTO J， YAMAMOTO D， 2019. Sex mysteries of the fly courtship master regulator fruitless ［J］. Fron Behav Neurosci， 13： 245.

SIGGS O M， BEUTLER B， 2012. The BTB-ZF transcription factors［J］. Cell Cycle， 11（18）： 3358-3369.

SIMON F， RAMAT A， LOUVET-VALLÉE S， et al， 2019. Shaping of Drosophila neural cell lineages through coordination of cell proliferation and cell fate by the BTB-ZF transcription factor tramtrack-69［J］. Genetics， 212（3）： 773-788.

WOHL M， ISHII K， ASAHINA K， 2020. Layered roles of fruitless isoforms in specification and function of male aggression-promoting neurons in Drosophila［J］. eLife， 9：e52702.

ZOLLMAN S， GODT D， PRIVÉ G G， et al， 1994. The BTB domain， found primarily in zinc finger proteins， defines an evolutionarily conserved family that includes several developmentally regulated genes in Drosophila［J］. Proc Nat Acad Sci USA， 91（22）： 10717-10721.

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Related Author

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YANG Mei

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LI Guanghong

WANG Fanghai

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Related Institution

State Key Laboratory for Biocontrol and Institute of Entomology / School of Life Sciences， Sun Yat-sen University

State Key Laboratory for Biocontrol and Institute of Entomology / School of Life Sciences， Sun Yat-sen Univercity

College of Forestry， Gansu Agricultural University

中山大学生命科学学院

Forestry College of Gansu Agricultural University

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The ttk gene structural characteristics of Sogatella furcifera and its expression level in nymphs

DOI：10.13471/j.cnki.acta.snus.2022E019

摘要

Abstract

关键词

Keywords

references