Immunomodulatory activity of crude exopolysaccharide from <italic style="font-style: italic">Parabacteroides distasonis</italic>

ZHU Yanglu; ZHANG Qian; LI Siju; CAO Xueqin; YE Simin; XIE Zhiyong

doi:10.13471/j.cnki.acta.snus.2021E053

您当前的位置：

首页 >

文章列表页 >

Immunomodulatory activity of crude exopolysaccharide from Parabacteroides distasonis

Research articles | 更新时间：2023-11-01

- Immunomodulatory activity of crude exopolysaccharide from Parabacteroides distasonis
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 61, Issue 6, Pages: 44-50(2022)
- 作者机构：
  
  中山大学药学院（深圳），广东深圳 518107
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2021E053
  CLC： R966
- Published：25 November 2022，
  
  Published Online：30 March 2022，
  
  Received：21 December 2021，
  
  Accepted：27 February 2022
扫描看全文
祝阳露,张倩,李四菊等.狄氏副拟杆菌胞外多糖粗提物的免疫调节活性[J].中山大学学报(自然科学版),2022,61(06):44-50.

ZHU Yanglu,ZHANG Qian,LI Siju,et al.Immunomodulatory activity of crude exopolysaccharide from Parabacteroides distasonis[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(06):44-50.
祝阳露,张倩,李四菊等.狄氏副拟杆菌胞外多糖粗提物的免疫调节活性[J].中山大学学报(自然科学版),2022,61(06):44-50. DOI： 10.13471/j.cnki.acta.snus.2021E053.

ZHU Yanglu,ZHANG Qian,LI Siju,et al.Immunomodulatory activity of crude exopolysaccharide from Parabacteroides distasonis[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(06):44-50. DOI： 10.13471/j.cnki.acta.snus.2021E053.

摘要

通过水提醇沉、Sevag试剂除蛋白、AB-8大孔吸附树脂脱色得到狄氏副拟杆菌

Parabacteroides distasonis

胞外多糖粗提物（PEP），并以巨噬细胞RAW 264.7为模型，采用MTT、Griess、DCFH、中性红实验和实时荧光定量PCR，对PEP的体外免疫调节活性进行探究。结果表明PEP为首次从狄氏副拟杆菌中提取到的胞外多糖，PEP是由甘露糖、鼠李糖、葡萄糖、半乳糖及岩藻糖按照19.35∶8.70∶5.24∶8.17∶1.00的比例组成的杂多糖，并且PEP显示以浓度依赖的方式促进巨噬细胞的增殖，增加巨噬细胞对NO、活性氧的释放，增强巨噬细胞的吞噬能力，并在基因水平上提高巨噬细胞中细胞因子（IL-1β、IL-6、TNF-α）的表达量，表明PEP具有良好的免疫调节能力，为PEP未来发展成为潜在的免疫刺激剂提供了一定的参考价值。

Abstract

Crude exopolysaccharide （PEP） from

Parabacteroides distasonis

was obtained by extraction in water， precipitation in ethanol， deproteinization using Sevag reagent and decolorization by AB-8 macroporous adsorption resin.Using RAW 264.7 macrophage as a model， the immunomodulatory xactivity of PEP was investigated by MTT assay， Griess assay， DCFH assay， neutral red experiment and real-time fluorescence quantitative PCR. The PEP was the exopolysaccharide extracted from

Parabacteroides distasonis

for the first time， and PEP was a heteropolysaccharide composed of mannose， rhamnose， glucose， galactose and fucose in a ratio of 19.35∶8.70∶5.24∶8.17∶1.00.Functional investigation showed that PEP could promote the proliferation of macrophages， increase the release of nitric oxide and reactive oxygen species by macrophages， enhance the phagocytosis ability of macrophages， and promote the production of cytokines （IL-1β，IL-6，TNF-α） by macrophages at the gene level in a concentration-dependent manner. The results suggested that PEP possessed good immunomodulatory ability， providing a certain reference value for the development of PEP as a potential immune stimulant in the future.

关键词

狄氏副拟杆菌Parabacteroides distasonis胞外多糖粗提物免疫调节活性

Keywords

Parabacteroides distasoniscrude exopolysaccharideimmunomodulatory activity

references

王俊永，姚蒙蒙，王晓冰，等. 益生菌胞外多糖的生物活性研究进展［J］. 饲料工业， 2020， 41（22）： 9-11.

JIANG B S， TIAN L L， HUANG X L， et al. Characterization and antitumor activity of novel exopolysaccharide APS of Lactobacillus plantarum WLPL09 from human breast milk［J］. International Journal of Biological Macromolecules， 2020， 163： 985-995.

李卫娜，赵波，柳陈坚，等. 副干酪乳杆菌胞外多糖抗氧化活性分析［J］. 食品工业科技， 2019， 40（24）： 34-39.

LIU J S， ZENG Y X， BI S Y， et al. Characterization and chemical modification of PLN-1， an　exopolysaccharide from Phomopsis liquidambari NJUSTb1［J］. Carbohydrate Polymers， 2021， 253： 117197.

SONAWANE H， ARYA S， GHOLE V， et al. Hypoglycemic and anticataract activity of crude exopolysaccharides of medicinal mushroom Phellinus badius on streptozotocin-induced diabetic rats and goat eye lenses respectively［J］. Bioactive Carbohydrates and Dietary Fibre， 2020， 24： 100241.

WANG K， LIAO M F， ZHOU N， et al. Parabacteroides distasonis alleviates obesity and metabolic dysfunctions via production of succinate and secondary bile acids［J］. Cell Reports， 2019， 26： 222-235.

DZIARSKI R， PARK S Y， KASHYAP D R， et al. Pglyrp-regulated gut microflora Prevotella falsenii， Parabacteroides distasonis and Bacteroides eggerthii enhance and Alistipes finegoldii attenuates Colitis in mice［J］. PLoS One， 2016， 11（1）： e0146162.

KOH G Y， KANE A V， WU X， et al. Parabacteroides distasonis attenuates tumorigenesis， modulates inflammatory markers and promotes intestinal barrier integrity in azoxymethane-treated A/J mice［J］. Carcinogenesis， 2020， 41（7）： 909-917.

CEKANAVICIUTEA E， YOOB B B， RUNIAA T F， et al. Gut bacteria from multiple sclerosis patients modulate human T cells and exacerbate symptoms in mouse models［J］. PNAS， 2017， 114（42）： e8943.

徐怀德，李晋，李钰金，等. 大孔吸附树脂脱除洋葱多糖色素技术研究［J］. 食品科学， 2012， 33（6）： 127-131.

张倩茹，凌蕾，何芋岐，等. 苯酚硫酸法测定苗药艾纳香中多糖含量［J］. 中国民族民间医药， 2019， 28（14）： 30-32.

WU M Q， LI W， ZHANG Y L， et al. Structure characteristics， hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate × Rosa rugosa waste［J］.Carbohydrate Polymers， 2021， 253： 117190.

伍芳芳. 猴头菇多糖的结构表征、免疫调节活性及其机理研究［D］. 广州：华南理工大学， 2018：81-83.

ZHANG Y， ZENG Y， CUI Y S， et al. Structural characterization， antioxidant and immunomodulatory activities of a neutral polysaccharide from Cordyceps militaris cultivated on hull-less barley［J］. Carbohydrate Polymers， 2020， 235： 115969.

YU Q， NIE S P， WANG J Q， et al. Toll-like receptor 4-mediated ROS signaling pathway involved in Ganoderma atrum polysaccharide-induced tumor necrosis factor-α secretion during macrophage activation［J］. Food and Chemical Toxicology， 2014， 66： 14-22.

GORDON S. Phagocytosis： the legacy of metchnikoff［J］. Cell， 2016， 166： 1065-1068.

TIAN J J， ZHANG C P， WANG X M. Structural characterization and immunomodulatory activity of intracellular polysaccharide from the mycelium of Paecilomyces cicadae TJJ1213［J］. Food Research International， 2021， 147： 110515.

WANG Y F， ZHANG Y F， SHAO J J， et al. Study on the immunomodulatory activity of a novel polysaccharide from the lichen Umbilicaria esculenta［J］. International Journal of Biological Macromolecules， 2019， 121： 846-851.

HORSSEN R V, HAGEN T L, EGGERMONT A M. TNF-α in cancer treatment: Molecular insights, antitumor effects, and clinical utility［J］. The Oncologist， 2006， 11： 397-408.

XU Z C， LIN R Y， HOU X N， et al. Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway ［J］. International Journal of Biological Macromolecules， 2020， 164： 4329-4338.

WANG H M， WANG X L， LI Y， et al. Structural properties and in vitro and in vivo immunomodulatory activity of an arabinofuranan from the fruits of Akebia quinata［J］. Carbohydrate Polymers， 2021， 256： 117521.

ZHANG W Y， YAN J Y， WU L H， et al. In vitro immunomodulatory effects of human milk oligosaccharides on murine macrophage RAW264.7 cells［J］. Carbohydrate Polymers， 2019， 207： 230-238.

HOTCHKISS R S， MONNERET G， PAYEN D. Immunosuppression in sepsis： A novel understanding of the disorder and a new therapeutic approach［J］. Lancet Infectious Diseases， 2013， 13（3）： 260-268.

ZHANG Y， ZENG Y， MEN Y， et al. Structural characterization and immunomodulatory activity of exopolysaccharides from submerged culture of Auricularia auricula-judae［J］. International Journal of Biological Macromolecules， 2018， 115： 978-984.

张鹏. 沼泽红假单胞菌胞外多糖的制备、结构表征及生物活性研究［D］. 咸阳：西北农林大学， 2019：36-44.

ELENA G G， SAXENA M， BHARDWAJ N. Modulation of innate immunity in the tumor microenvironment ［J］. Cancer Immunology Immunotherapy， 2016， 65（10）： 1261-1268.

ZHANG X Y， LIU Z J， ZHONG C， et al. Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra ［J］. International Journal of Biological Macromolecules， 2021， 189： 980-992.

SONG X F， REN T， ZHENG Z. Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-κB signaling pathway［J］. International Journal of Biological Macromolecules， 2017， 95： 357-362.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

Yanglu ZHU

Qian ZHANG

Siju LI

Xueqin CAO

Simin YE

Zhiyong XIE

Related Institution

No data

Postal code：510275
Tel：020-84112585，84113223 Email：xuebaozr@mail.sysu.edu.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Immunomodulatory activity of crude exopolysaccharide from Parabacteroides distasonis

DOI：10.13471/j.cnki.acta.snus.2021E053

摘要

Abstract

关键词

Keywords

references