Species diversity of endophytic fungi from <italic style="font-style: italic">Anemarrhena asphodeloides</italic> and their antagonistic activity <italic style="font-style: italic">in vitro</italic> against superficial pathogenic fungi

LI Jiaqi; HU Haiyan; YAO Huaxiong; LI Jianbin; CHEN Meiqi; WANG Junyan; ZHOU Xiaoling; DENG Zujun

doi:10.13471/j.cnki.acta.snus.2021E041

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Species diversity of endophytic fungi from Anemarrhena asphodeloides and their antagonistic activity in vitro against superficial pathogenic fungi

Research articles | 更新时间：2023-11-01

- Species diversity of endophytic fungi from Anemarrhena asphodeloides and their antagonistic activity in vitro against superficial pathogenic fungi
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 61, Issue 5, Pages: 84-93(2022)
- 作者机构：
  
  1.广东药科大学生命科学与生物制药学院 / 广东省生物活性药物研究重点实验室，广东广州510006
  2.广州市微生物研究所，广东广州 510663
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2021E041
  CLC： R289;R22;R33
- Published：25 September 2022，
  
  Published Online：21 January 2022，
  
  Received：28 September 2021，
  
  Accepted：15 December 2021
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李佳琪,胡海艳,姚华雄等.知母内生真菌多样性及其对浅表感染真菌的体外拮抗活性[J].中山大学学报(自然科学版),2022,61(05):84-93.

LI Jiaqi,HU Haiyan,YAO Huaxiong,et al.Species diversity of endophytic fungi from Anemarrhena asphodeloides and their antagonistic activity in vitro against superficial pathogenic fungi[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(05):84-93.
李佳琪,胡海艳,姚华雄等.知母内生真菌多样性及其对浅表感染真菌的体外拮抗活性[J].中山大学学报(自然科学版),2022,61(05):84-93. DOI： 10.13471/j.cnki.acta.snus.2021E041.

LI Jiaqi,HU Haiyan,YAO Huaxiong,et al.Species diversity of endophytic fungi from Anemarrhena asphodeloides and their antagonistic activity in vitro against superficial pathogenic fungi[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(05):84-93. DOI： 10.13471/j.cnki.acta.snus.2021E041.

摘要

分析药用植物知母

Anemarrhena asphodeloides

根、茎、叶中内生真菌的多样性及其次级代谢产物对浅表感染真菌的抑菌活性，为寻找皮肤真菌病的潜在药物奠定基础。从河北省太行山采集到5株健康的野生知母，采用组织块法分离根、茎、叶的内生真菌；结合形态学和分子生物学方法分类鉴定内生真菌；并分别采用菌丝生长抑制速率法和琼脂扩散法筛选对5种浅表感染真菌具有抑制活性的菌株。本研究从知母中共分离获得438株内生真菌，隶属于23个属。根、茎、叶的真菌定殖率分别为99.33%、74.00%和47.33%，其中根部的优势类群为镰刀菌属

Fusarium

（60.29%）和棘壳孢属

Setophoma

（33.97%）；茎部的优势类群为镰刀菌属（91.50%）和粘帚霉属

Clonostachys

（7.19%）；叶部的优势类群为镰刀菌属（35.53%）和链格孢属

Alternaria

（14.47%）。香农多样性指数显示，叶中的物种多样性最高，其次是根和茎。对30株代表性菌株发酵液的抑菌实验结果表明：对犬小孢子菌

Microsporum canis

、红色毛癣菌

Trichophyton rubrum

、黏膜毛孢子菌

Trichosporon mucoides

、糠秕马拉色菌

Malassezia furfur

和白色念珠菌

Candida albicans

具有抑制活性的菌株分别占总数的96.67%、100.00%、100.00%、3.33%和3.33%，其中菌株ZMR35对4种浅表感染真菌均具较强的抑制活性，菌株ZML33则对犬小孢子菌、红色毛癣菌、黏膜毛孢子菌的抑制率均高于70%，经分子鉴定两者分别属于黑团孢属

Periconia

和曲霉属

Aspergillus

。知母蕴含较为丰富的内生真菌资源，其中还有较高比例菌株的发酵产物对浅表感染真菌表现出良好的拮抗活性，这为浅表感染真菌病药物的筛选提供了潜在资源库。

Abstract

The study investigated the diversity of endophytic fungi in the roots， stems and leaves of the medicinal plant

Anemarrhena asphodeloides

and the antimicrobial activity of their secondary metabolites provide references for discovering new potent antimycotics against opportunistic pathogenic yeasts and dermatophytes. Samples of roots， stems and leaves of 5 healthy wild

A. asphodeloides

plants were collected from the Taihang Mountain in Hebei Province. The endophytic fungi of

A.asphodeloides

were isolated by tissue block isolation method，and were identified by morphological and molecular biological methods.The antimicrobial activity of opportunistic pathogenic yeasts and dermatophytes of endophytic fungi were studied by the agar diffusion and mycelial growth inhibition methods， respectively. A total of 438 isolates of endophytic fungi were isolated from

A. asphodeloides

tissues，these isolates belong to 23 genera. The colonization rate of the endophytic fungi in the roots， stems and leaves were 99.33%， 74.00% and 47.33% respectively.

Fusarium

（60.29%）and

Setophoma

（33.97%）were the dominant genera in the roots，

Fusarium

（91.50%）and

Clonostachys

（7.19%）were the dominant genera in the stems， and

Fusarium

（35.53%）and

Alternaria

（14.47%）were the dominant genera in the leaves. The Shannon diversity index shows that the species diversity in leaves is the highest， followed by roots and stems. The antibacterial results of 30 representative strains by performing fermentation broth showed that： 96.77%， 100.00%， 100.00%， 3.33% and 3.33% of the isolated endophytic fungi had inhibitory activity against

Microsporum canis， Trichophyton rubrum， Trichosporon mucoides， Malassezia furfur

and

Candida albicans

，

respectively. Among them， the strain ZMR35 has a strong inhibitory activity against four kinds of superficial fungi. The inhibition rates of the fermentation broth of strain ZML33 against

Microsporum canis， Trichophyton rubrum， Trichosporon mucoides

were all higher than 70%. The results indicated that the species diversity of

A. asphodeloides

endophytic fungi was rich and the colonization of these species showed tissue specificity. Two of the strains ZMR35 and ZML33 exhibited significant inhibitory effects on a variety of superficial pathogenic fungi

in vitro

. Our results indicate that the secondary metabolites produced by the endophytic fungi of

A. asphodeloides

can be used as natural drug candidates for the treatment of superficial mycosis.

关键词

知母Anemarrhena asphodeloides内生真菌多样性浅表感染真菌抑菌活性

Keywords

Anemarrhena asphodeloidesendophytic fungidiversitysuperficial pathogenic fungiantimicrobial activities

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Related Institution

College of Materials and Energy， South China Agricultural University / Key Laboratory for Biobased Materials and Energy of Ministry of Education

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中山大学环境科学研究所

College of Life Science , Guangxi Normal University

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Species diversity of endophytic fungi from Anemarrhena asphodeloides and their antagonistic activity in vitro against superficial pathogenic fungi

DOI：10.13471/j.cnki.acta.snus.2021E041

摘要

Abstract

关键词

Keywords

references