Structural features of DNMT1 gene and its differential expression between male and female adults of <italic style="font-style: italic">Sogatella furcifera</italic>

HE Jingyi; YANG Mei; LIN Jia; LIANG Anwen; LI Guanghong; WANG Fanghai

doi:10.13471/j.cnki.acta.snus.2020E051

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Structural features of DNMT1 gene and its differential expression between male and female adults of Sogatella furcifera

Research articles | 更新时间：2023-11-01

- Structural features of DNMT1 gene and its differential expression between male and female adults of Sogatella furcifera
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 61, Issue 2, Pages: 76-81(2022)
- 作者机构：
  
  有害生物控制与资源利用国家重点实验室 / 中山大学生命科学学院，广东广州 510275
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2020E051
  CLC： Q966
- Published：25 March 2022，
  
  Published Online：23 April 2021，
  
  Received：29 November 2020，
  
  Accepted：19 January 2021
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何静怡,杨梅,林佳等.白背飞虱DNMT1基因的结构特点及在雌雄成虫中的差异表达[J].中山大学学报(自然科学版),2022,61(02):76-81.

HE Jingyi,YANG Mei,LIN Jia,et al.Structural features of DNMT1 gene and its differential expression between male and female adults of Sogatella furcifera[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(02):76-81.
何静怡,杨梅,林佳等.白背飞虱DNMT1基因的结构特点及在雌雄成虫中的差异表达[J].中山大学学报(自然科学版),2022,61(02):76-81. DOI： 10.13471/j.cnki.acta.snus.2020E051.

HE Jingyi,YANG Mei,LIN Jia,et al.Structural features of DNMT1 gene and its differential expression between male and female adults of Sogatella furcifera[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(02):76-81. DOI： 10.13471/j.cnki.acta.snus.2020E051.

摘要

DNA甲基转移酶1（DNMT1）是基因组DNA甲基化关键酶之一，在生物的生长发育过程中起到重要的调控作用。本研究在白背飞虱（

Sogatella furcifera

）转录组测序结果中找到一条带有起始密码子ATG和终止密码子TGA的DNMT1基因的cDNA序列，全长为4 524 bp，编码的蛋白质具有DNMT1的典型结构：位于N端的DNMT相关蛋白结合结构域，复制灶靶向序列，富含半胱氨酸的锌指结构域和聚溴同源结构域，以及位于C端的催化结构域。进一步与白背飞虱全基因组序列进行比对，发现白背飞虱DNMT1基因位于白背飞虱第3号染色体上，全长为22 567 bp，含有18个外显子和17个内含子。该基因主要在白背飞虱腹部表达，且在雌成虫中的表达量明显高于雄成虫，达4.3倍。本研究将增进对昆虫DNMTs的具体结构和生物学功能的认识，并为探索新的防治稻飞虱的方法或途径提供理论基础。

Abstract

DNA methyltransferase 1 （DNMT1） is one of the key enzymes for genomic DNA methylation and plays an important regulatory role in the growth and development of organisms. We obtained a cDNA sequence of DNMT1 from the transcriptome of

Sogatella furcifera

， and then analyzed its structure. The transcript has an open reading frame of 4 524 bp in length containing an initiation codon ATG and a termination codon TGA. The protein encoded has the typical structure of DMAP binding domain， replication foci targeting sequence， CXXC zinc finger domain and Polybromo homology domain located at the N-terminal and Dcm domain located at the C-terminal. Comparing the sequence with the whole genome sequence of

S. furcifera

， the DNMT1 gene was found locating on chromosome 3 of

S. furcifera

， with a full length of 22 567 bp， containing 18 exons and 17 introns. The gene was mainly expressed in the abdomen of the white-backed planthopper， and the expression level in female adults was significantly higher than that in male adults， up to 4.3 times. This study could enhance the understanding of the specific structure and biological function of insect DNMTs and provide a theoretical basis for the exploration of new methods or pathways for the control of planthopper.

关键词

DNMT1白背飞虱（Sogatella furcifera）开放阅读框基因表达

Keywords

DNMT1Sogatella furciferaopen reading framegene expression

references

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Related Author

Mei YANG

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Related Institution

State Key Laboratory for Biocontrol and Institute of Entomology / School of Life Sciences， Sun Yat-sen Univercity

State Key Laboratory for Biocontrol and Institute of Entomology， School of Life Sciences， Sun Yat-sen University

College of Forestry， Gansu Agricultural University

中山大学生命科学学院

Forestry College of Gansu Agricultural University

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Structural features of DNMT1 gene and its differential expression between male and female adults of Sogatella furcifera

DOI：10.13471/j.cnki.acta.snus.2020E051

摘要

Abstract

关键词

Keywords

references