Discovery of the active ingredients and the mechanism of action for the effects of Honghe Fujie Lotion on infection based on network pharmacology methods

RAO Hongyu; LI Peibo; CHEN Pan; SHI Zhiheng; SU Weiwei

doi:10.13471/j.cnki.acta.snus.2020E032

您当前的位置：

首页 >

文章列表页 >

Discovery of the active ingredients and the mechanism of action for the effects of Honghe Fujie Lotion on infection based on network pharmacology methods

Research articles | 更新时间：2023-11-01

- Discovery of the active ingredients and the mechanism of action for the effects of Honghe Fujie Lotion on infection based on network pharmacology methods
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 61, Issue 3, Pages: 21-27(2022)
- 作者机构：
  
  广东省中药上市后质量与药效再评价工程技术研究中心 / 广东省热带亚热带植物资源重点实验室 / 中山大学生命科学学院，广东广州 510275
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2020E032
  CLC： R285
- Published：25 May 2022，
  
  Published Online：20 May 2021，
  
  Received：31 July 2020，
  
  Accepted：14 October 2020
扫描看全文
饶鸿宇,李沛波,谌攀等.基于网络药理学探讨红核妇洁洗液抗感染的作用机制[J].中山大学学报(自然科学版),2022,61(03):21-27.

RAO Hongyu,LI Peibo,CHEN Pan,et al.Discovery of the active ingredients and the mechanism of action for the effects of Honghe Fujie Lotion on infection based on network pharmacology methods[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(03):21-27.
饶鸿宇,李沛波,谌攀等.基于网络药理学探讨红核妇洁洗液抗感染的作用机制[J].中山大学学报(自然科学版),2022,61(03):21-27. DOI： 10.13471/j.cnki.acta.snus.2020E032.

RAO Hongyu,LI Peibo,CHEN Pan,et al.Discovery of the active ingredients and the mechanism of action for the effects of Honghe Fujie Lotion on infection based on network pharmacology methods[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(03):21-27. DOI： 10.13471/j.cnki.acta.snus.2020E032.

摘要

采用网络药理学方法分析红核妇洁洗液在抗感染过程中发挥功效的潜在靶点和通路，为红核妇洁洗液的机制研究提供参考。首先运用TCMSP、SwissTargetPrediction和CTD数据库检索得到红核洗液中92个化合物的潜在靶标，再检索DisGeNET数据库获得感染性疾病相关靶点，将两者整合去重，得到与抗感染相关的交集靶点合计143个。构建药材-成分-靶点网络后，采用Cytoscape研究该网络的拓扑结构，明确红核妇洁洗液抗感染的关键化合物和潜在作用靶标，主要涉及PARP1、PTGS1、ESR1、IDO1、NOS2、ALOX5、EGFR、PTGS2、HDAC6和JAK1等。将交集靶点导入David数据库进行分析，发现红核洗液主要通过脂多糖介导的通路、TNF 通路、TLR通路和NF-kappa B 通路等发挥抗感染的功效。此外，红核洗液中还能抑制病原菌入侵和繁殖、调节机体免疫应答、减轻炎症反应、缓解机体氧化应激损伤，从而发挥抗感染的作用。

Abstract

The study aimed to explore the mechanism of anti-infection of Honghe Fujie Lotion （HHFJL） through network pharmacology， and provide a scientific reference for its clinical application. 92 compounds in HHFJL were identified by GC-MS in preliminary studies. The action targets were collected by TCMSP， Toxicogenomics Database （CTD） and SwissTargetPrediction， while the infection related targets were queried through DisGeNET database， and the “compound-disease” intersection targets were obtained using Venn Diagram. The herb- active ingredient- intersection target network was constructed and analysized by Cytoscape. A total of 1 056 targets were collected for HHFJL， and 143 key targets were selected， such as PARP1， PTGS1， ESR1， IDO1， NOS2， ALOX5， EGFR， PTGS2， HDAC6， JAK1， and so on. The results from DAVID indicated that lipopolysaccharide-mediated signaling pathway， NF-kappa B signaling pathway， Toll-like receptor signaling pathway and TNF signaling pathway were the key pathways， which were involved in anti-infective therapies. HHFJL may show therapeutic effects on infection probably through inhibiting pathogenic， regulating immune function， alleviating inflammation and oxidative damage based on multi-target and multi-pathway.

关键词

红核妇洁洗液抗感染网络药理学

Keywords

Honghe Fujie Lotioninfectionnetwork pharmacology

references

熊英，黄服喜. 红核妇洁洗液含量测定方法的改进［J］. 中国药品标准， 2005，6（5）：54-55.

王鹤，韩学学，郎桂清，等. 红核妇洁洗液联合硝呋太尔制霉素阴道软胶囊治疗妊娠期霉菌性阴道炎的疗效观察［J］. 中国医院用药评价与分析， 2015， 15（3）：306-308.

李秀兰，杨铭越，齐晓玲. 红核妇洁洗液预防阴式手术切口感染的临床观察［J］. 现代中西医结合杂志， 2010， 19（32）：4130-4131.

梅龙，林蓉，卢朝辉. 红核妇洁洗液体外抗菌实验研究［J］. 中国妇产科临床杂志， 2014， 15（2）：145-147.

靳真，姚凤，商晨旭，等. 红核妇洁洗液对白念珠菌生物膜及浅部真菌体外作用研究［J］. 中国临床药理学杂志， 2017， 33（16）：1556-1560.

RAO H Y， LI P B， WU H， et al. Simultaneous determination of six compounds in destructive distillation extracts of hawthorn seed by GC-MS and evaluation of their antimicrobial activity ［J］. Molecules， 2019， 24（23）：4328.

饶鸿宇，朱承科，彭维，等. 红核妇洁洗液化学物质基础研究［J］. 药学研究， 2020， 39（3）：133-137.

饶鸿宇，李沛波，谌攀，等. 基于网络药理学探讨红核妇洁洗液抗炎的活性成分和作用机制［J］.药学研究，2021，40（1）： 9-15.

刘忠政，梁洁萍，聂怡初，等. 复方血栓通胶囊基于血液循环和凝血过程相关靶点的网络药理学研究［J］. 中山大学学报（自然科学版）， 2013， 52（2）：97-100.

李泮霖，苏薇薇. 网络药理学在中药研究中的最新应用进展［J］.中草药，2016，47（16）： 2938-2942.

李泮霖，刘宏，廖弈秋，等. 基于网络药理学探讨丹红注射液成分-抗血栓靶点的相互作用［J］. 中山大学学报（自然科学版）， 2018， 57（4）：121-127.

刘梓燊，杨国旺. 基于网络药理学分析“黄芪―白术”治疗肺癌的作用机制［J］. 中山大学学报（自然科学版）， 2020， 59（5）：134-143.

程锦泉，刘少础. 超级细菌的警示与滥用抗生素潜在公共卫生问题［J］. 中国公共卫生， 2010， 26（12）：1521-1522.

MARCHESE A， BARBIERI R， COPPO E， et al. Antimicrobial activity of eugenol and essential oils containing eugenol： A mechanistic viewpoint ［J］. Critical Reviews in Microbiology， 2017， 43（6）：668-689.

BHATIA R， SHREAZ S， KHAN N， et al. Proton pumping ATPase mediated fungicidal activity of two essential oil components ［J］. Journal of Basic Microbiology， 2012， 52（5）：504-512.

FONTES PINHEIRO P， ALVES PARREIA MENINI L， CAMPOS BERNARDS P， et al. Semisynthetic phenol derivatives obtained from natural phenols： Antimicrobial activity and molecular properties ［J］. Journal of Agricultural and Food Chemistry，2018，66（1）：323-330.

KOCACALISKAN I， TALAN I， TERZI I. Chemical composition， antioxidant， and antibacterial activity of wood vinegar from Litchi chinensis［J］. Molecules，2016， 21， 1150.

杨梅，江丹，易筠，等. 艾叶燃烧物清除自由基作用的观察［J］. 中国针灸， 2009，29（7）：547-549.

WESTERA L， JENNINGS M A， MAAMARY J， et al. Poly-ADP ribosyl polymerase 1 （PARP1） regulates influenza a virus polymerase ［J］. Advances in Virology，2019（18）：1-11.

SHOU Q Y， FU H Y， HUANG X P， et al. PARP-1 controls NK cell recruitment to the site of viral infection ［J］. JCI Insight， 2019， 4（12）：e121291.

WANG C， DU M， HUANG D， et al. Inhibition of PARP1 increases IRF-dependent gene transcription in Jurkat cells ［J］. Current Medical Science， 2019， 39（3）：356-362.

BLANCA LOPEZ N， SORIANO V， GARCIA MARTIN E， et al. NSAID-induced reactions： Classification， prevalence， impact， and management strategies ［J］. Journal of Asthma and Allergy， 2019， 12：217-233.

GIMENEZ G， BELAUNZARÁN M L， MAGALHÃES K G， et al. Involvement of TLR6 in the induction of COX-2， PGE2 and IL-10 in macrophages by lipids from virulent S2P and attenuated R1A Babesia bovis strains ［J］. Veterinary Parasitology， 2016， 223：127-132.

HEMMAT N， BANNAZADEH BAGHI H. Association of human papillomavirus infection and inflammation in cervical cancer ［J］. Pathogens and Disease， 2019，77（5）： ftz048.

LASSO G， MAYER S V， WINKELMANN E R， et al. A structure-informed Atlas of human-virus interactions ［J］. Cell， 2019， 178（6）：1526-1541.

MANCHALA N R， DUNGDUNG R， TRIVEDI P， et al. Mycophenolic acid （MPA） modulates host cellular autophagy progression in sub genomic dengue virus-2 replicon cells ［J］. Microbial Pathogenesis， 2019，137：103762.

SALINAS MUNOZ L， CAMPOS FERNANDEZ R， MERCADER E， et al. Estrogen receptor-alpha （ESR1） governs the lower female reproductive tract vulnerability to Candida albicans ［J］. Frontiers in Immunology， 2018，9：1033.

ZIKLO N， HUSTON W M， TAING K， et al. High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis， are independent of host IFN-γ response ［J］. BMC Infectious Diseases， 2019， 19（1）：218.

ROCHA D M， CALDAS A P， OLIVEIRA L L， et al. Saturated fatty acids trigger TLR4-mediated inflammatory response［J］. Atherosclerosis， 2016， 244：211-215.

CHU C Q. How much have we learnt about the TNF family of cytokines？［J］. Cytokine， 2018， 101： 1-3.

YADAV N， CHANDRA H. Modulation of alveolar macrophage innate response in proinflammatory-， pro-oxidant-， and infection- models by mint extract and chemical constituents： Role of MAPKs ［J］. Immunobiology， 2018， 223（1）：49-56.

LI Y H， ZHOU W H， TAO Y， et al. The Galectin-9/Tim-3 pathway is involved in the regulation of NK cell function at the maternal-fetal interface in early pregnancy ［J］. Cellular & Molecular Immunology， 2016， 13（1）：73-81.

LUAN H， ZHANG Q， WANG L， et al. OM85-BV induced the productions of IL-1β， IL-6， and TNF-α via TLR4- and TLR2-mediated ERK1/2/NF-κB pathway in RAW264.7 cells ［J］. Journal of Interferon & Cytokine Research， 2014， 34（7）： 526-536.

YU Q， WANG Y F， DONG L， et al. Regulations of glycolytic activities on macrophages functions in tumor and infectious inflammation ［J］. Frontiers in Cellular and Infection Microbiology， 2020， 10：287.

PRADHAN A， AVELAR G M， BAIN J M， et al. Hypoxia promotes immune evasion by triggering β-glucan masking on the Candida albicans cell surface via mitochondrial and cAMP-protein kinase a signaling ［J］. mBio， 2018， 9（6）：e01318-18.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Exploring the mechanism and function of Xiasangju in the treatment of xerophthalmia based on network pharmacology and experimental validation

Network pharmacology analysis of Compound Xueshuantong Capsule （CXC） in improving microcirculation

Discovery of the active ingredients and the mechanism of action for the effects of Bufei Huoxue Capsule on pneumonia recovery based on network pharmacology methods

Mechanisms of “Astragalus membranaceus - Atractylodes macrocephala koidz” for the treatment of lung cancer based on network pharmacology

Related Author

Weiwei SU

Zhiheng SHI

Pan CHEN

Peibo LI

Hongyu RAO

SUN Weiguang

LI Jinliang

HU Shuangfei

Related Institution

Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-evaluation of Post-marketed TCM / Guangdong Key Laboratory of Plant Resources / School of Life Sciences， Sun Yat-sen University

Guangzhou Baiyunshan Xingqun Pharmaceutical Co.， Ltd

School of Life Sciences， Sun Yat-sen University / Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-evaluation of Post-marketed TCM

Department of Oncology，Beijing Hospital of Traditional Chinese Medicine， Capital Medical University

Capital Medical University

Postal code：510275
Tel：020-84112585，84113223 Email：xuebaozr@mail.sysu.edu.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰