The mechanism of <italic style="font-style: italic">Suaeda rigida</italic> polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus

SHA Ailong; HAO Haiyan

doi:10.13471/j.cnki.acta.snus.2020.05.12.2020E016

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The mechanism of Suaeda rigida polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus

更新时间：2023-11-01

- The mechanism of Suaeda rigida polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 60, Issue 4, Pages: 19-25(2021)
- 作者机构：
  
  1.重庆三峡学院教师教育学院，重庆 404120
  2.重庆三峡学院生物与食品工程学院 / 重庆市渝东北特色生物资源开发利用工程技术研究中心，重庆 404120
  3.重庆三峡学院环境与化学工程学院 / 三峡库区水环境演变与污染防治重庆市重点实验室，重庆 404120
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2020.05.12.2020E016
  CLC： R965;R285.5;Q955
- Published：25 July 2021，
  
  Published Online：28 October 2020，
  
  Received：12 May 2020，
  
  Accepted：23 July 2020
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沙爱龙,郝海燕.硬枝碱蓬多糖通过肠肌间神经丛抑制家兔离体十二指肠收缩及机制[J].中山大学学报(自然科学版),2021,60(04):19-25.

SHA Ailong,HAO Haiyan.The mechanism of Suaeda rigida polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2021,60(04):19-25.
沙爱龙,郝海燕.硬枝碱蓬多糖通过肠肌间神经丛抑制家兔离体十二指肠收缩及机制[J].中山大学学报(自然科学版),2021,60(04):19-25. DOI： 10.13471/j.cnki.acta.snus.2020.05.12.2020E016.

SHA Ailong,HAO Haiyan.The mechanism of Suaeda rigida polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2021,60(04):19-25. DOI： 10.13471/j.cnki.acta.snus.2020.05.12.2020E016.

摘要

研究了不同剂量的硬枝碱蓬多糖对家兔离体十二指肠收缩活动的影响，并探究其作用机制。利用Medlab生物信号采集处理系统记录用药前后各组家兔离体十二指肠的收缩活动，观察硬枝碱蓬多糖低、中、高三个剂量组对肠收缩频率和幅度的影响。分别选取Ach、CaCl

与高剂量硬枝碱蓬多糖共同孵育，观察对十二指肠收缩的影响。用ELISA法检测不同剂量的硬枝碱蓬多糖对家兔离体十二指肠中亮氨酸脑啡肽（Leu-enk）、甲硫氨酸脑啡肽（Met-enk）含量和酪氨酸羟化酶（TH）活性的影响。结果显示，与给药前相比，硬枝碱蓬多糖3个剂量组对家兔离体十二指肠收缩频率和幅度均具有抑制作用，其中低剂量组抑制不显著（

0.05）、中剂量组抑制显著（

0.05）、高剂量组抑制极显著（

0.01），均呈现一定的剂量依赖效应。高剂量硬枝碱蓬多糖均能极显著（

0.01）抑制Ach、CaCl

分别对肠收缩频率和幅度的促进作用，抑制效果分别与硫酸阿托品和盐酸维拉帕米相当。ELISA实验表明，与正常对照组相比，硬枝碱蓬多糖3个剂量组家兔离体十二指肠中Leu-enk和Met-enk含量均不同程度减少，TH活性均不同程度升高。上述结果提示，硬枝碱蓬多糖能抑制家兔离体十二指肠收缩，作用机制可能是通过肠肌间神经丛抑制G蛋白耦联M受体介导的AC- cAMP- PKA和PLC-IP

- Ca

信号转导通路及Ca

信号系统（如抑制肌膜I

Ca-L

进而抑制Ca

- CaM信号通路）；可能通过抑制肌间神经丛运动神经元释放Leu-enk和Met-enk，从而抑制G蛋白耦联δ受体介导的GTP-cAMP-（PKK或PKC）信号通路及Ca

信号系统；并可能通过促进肌间神经丛运动神经元释放TH引起NE增多，再激活G蛋白耦联β受体介导的AC- cAMP- PKA信号转导通路。

Abstract

The study aims to investigate the effect of different dosages of

Suaeda rigida

polysaccharides on the contractile activity of the isolated rabbit duodenum and explore its mechanism. The contractile activity of the isolated duodenum was recorded by Medlab biological signal acquisition and processing system before and after administration， and the effects of

Suaeda rigida

polysaccharides-treated groups of the low， medium， and high doses on the contractile frequency and amplitude of the intestines were observed. Acetylcholine （ACh） and CaCl

were selected respectively to be co-incubated with the high dose of

Suaeda rigida

polysaccharides to observe the effects on the duodenal contraction. The effects of different doses of

Suaeda rigida

polysaccharides on the contents of Leu enkephalin （Leu-enk）， met enkephalin （Met-enk） and the activity of tyrosine hydroxylase （TH） in the isolated rabbit duodenum were detected by enzyme-linked immunosorbent assay （ELISA）. The results showed that the three dose groups of

Suaeda rigida

polysaccharides had inhibitory effects on the contractile frequency and amplitude of the isolated duodenum in rabbits compared with those before administration. Among them， the inhibition of the low dose group was not significant （

0.05）， the medium dose group was significant （

0.05）， and the high dose group was very significant （

0.01）， showing a dose-dependent effect. The high dose of

Suaeda rigida

polysaccharides could significantly （

0.01） inhibit the promotion effects of ACh and CaCl

on the frequency and amplitude of intestinal contraction， and the inhibitory effects were similar to those of atropine sulfate and verapamil hydrochloride， respectively. The results of ELISA showed that compared with the control group， the contents of Leu-enk and Met-enk in the isolated rabbits duodenum in the three groups of

Suaeda rigida

polysaccharides were decreased in varying degrees， and the activity of TH was increased in varying degrees. These results indicated that

Suaeda rigida

polysaccharides can inhibit the contraction of the isolated rabbit duodenum. The mechanism may be that it can inhibit the signal transduction pathways of AC-cAMP-PKA and PLC-IP

-Ca

and Ca

signaling systems mediated by G protein-coupled M receptor through the intestinal myenteric plexus； it may inhibit the GTP-cAMP-（PKK or PKC） signaling pathways and Ca

signaling systems mediated by G protein-coupled δ receptor by inhibiting the release of Leu-enk and Met-enk from the motor neurons of the myenteric plexus； it may also cause the increase of NE by promoting the release of TH from the motor neurons of the myenteric plexus and reactivate the AC-cAMP- PKA signaling pathway mediated by the G protein-coupled β receptor.

关键词

硬枝碱蓬多糖十二指肠平滑肌肌间神经丛神经递质

Keywords

Suaeda rigida polysaccharidesduodenal smooth musclemyenteric plexusneurotransmitter

references

冯缨，严成，尹林克.新疆植物特有种及其分布［J］.西北植物学报，2003，23（2）：263-273.

FENG Y， YAN C， YIN L K. The endemic species and distribution in Xinjiang ［J］. Acta Bot Boreal-Occident Sin， 2003， 23（2）： 263-273.

郝海燕，沙爱龙．硬枝碱蓬化学成分的定性分析及生物碱含量的测定［J］．食品科技，2013，38（1）：244-247．

HAO H Y， SHA A L. The qualitative determination of chemical constituents and alkaloids in Suaeda rigida ［J］. Food Science and Technology， 2013， 38（1）： 244-247.

郝海燕，沙爱龙，骆春敏．硬枝碱蓬对卡拉库尔羊血清中谷胱甘肽及其相关酶活性的影响［J］.新疆农业大学学报，2017，40（1）：20-24．

HAO H Y， SHA A L， LUO C M. Effects of Suaeda rigida on glutathione and its related enzymes in the serum of karakul sheep ［J］. Journal of Xinjiang Agricultural University， 2017， 40（1）： 20-24.

沙爱龙，李继兴，葛建军，等．硬枝碱蓬对卡拉库尔羊生长性能和血液生理生化指标的影响［J］．西北农业学报，2013，22（3）：16-22．

SHA A L， LI J X， GE J J， et al. Effects of Suaeda rigida on growth performance and hematological and biochemical parameters of karakul sheep ［J］. Acta Agriculturae Boreali-Occidentalis Sinica， 2013， 22（3）： 16-22.

沙爱龙，杨雪萍，王伟，等．硬枝碱蓬多糖对免疫抑制小鼠血清中NO含量及NOS活性的影响［J］. 四川动物，2013，32（1）：90-92．

SHA A L， YANG X P， WANG W， et al. Effects of the Suaeda rigida polysaccharides on NO content and NOS activity in the serum of immunosuppressed mice ［J］. Sichuan Journal of Zoology， 2013， 32（1）： 90-92.

王东，袁昌鲁，林力，等．车前子多糖对小肠运动障碍小鼠的影响［J］．中华中医药学刊，2008，26（6）：1188-1189．

WANG D， YUAN C L， LIN L， et al. Effects of the polysaccharides in seeds of plantaginis on small intestinal motility disturbance in mice ［J］. Chinese Archives of Traditional Chinese Medicine， 2008， 26（6）： 1188-1189.

李兰城．锁阳多糖对小鼠胃排空、小肠推进功能的影响［J］．北方药学， 2013，10（9）：43-44．

LI L C. Effect of cynomorium songaricum polysaccharide on mice of gastric emptying and intestinal propulsion ［J］. Journal of North Pharmacy， 2013， 10（9）： 43-44.

王庭槐．生理学［M］．9版．北京：人民卫生出版社，2018：51-56，177-180．

WANG T H. Physiology ［M］. 9th ed. Beijing： People's Medical Publishing House， 2018： 51-56， 177-180.

SANDERS K M. A case for interstital cells of cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract ［J］. Gastroenterology， 1996， 111： 492-512.

王聪，赵盼，周亚男，等．黄酒通过肠肌间神经丛抑制大鼠离体小肠的收缩［J］．食品科学，2019，40（9）：173-178．

WANG C， ZHAO P， ZHOU Y N， et al. Chinese rice wine inhibits contraction activity of the isolated rat small intestine through intestinal myenteric plexus ［J］. Food Science， 2019， 40（9）： 173-178.

FURNESS J B. Integrated neural and endocrine control of gastrointestinal function［M］// BRIERLEY S， COSTA M. The enteric nervous system. Berlin： Springer International Publishing， 2016： 159-173.

黄崇生，吴辉雨，李阳友，等．火麻仁水煎液对兔离体小肠平滑肌收缩活动的影响及机制探究［J］．中医药临床杂志，2019，31（7）：1312-1316．

HUANG C S， WU H Y， LI Y Y， et al. Effect of huomaren decoction on contraction mechanism of rabbit small intestinal smooth muscle ［J］. Clinical Journal of Traditional Chinese Medicine， 2019， 31（7）： 1312-1316.

WALLACE J A， LI F， BALAKRISHNAN S， et al. Ets2 in tumor fibroblasts promotes angiogenesis in breast cancer ［J］. PLoS One， 2013， 8 （8）： e71533.

SHA A L， SUN H S， WANG Y Y. Immunohistochemical observations of methionine-enkephalin and delta opioid receptor in the digestive system of Octopus ocellatus ［J］. Tissue Cell， 2013， 45（1）： 83-87.

CHAKRABARTI S， WANG L， TANG W J， et al. Chronic morphine augment sadenylyl cyclase phosphorylation： relevance to altered signaling during tolerance/dependence ［J］. Mol Pharmacol， 1998， 54（6）： 949-953.

HEAGY W， TENG E， LOPEZ P， et al. Enkephalin receptors and receptor-mediated signal transduction in cultured human lymphocyte ［J］. Cell Immunology， 1999， 191（1）： 34-48.

MARTIN K I， GABRILOVAC J. The effect of δ-opioid agonists on intracellular calcium level in MOLT-4 T-cell line ［J］. International Journal of Immunopathology and Pharmacology， 1999， 12（3）： 113-119.

ARMAZ A， ADAMA B， JEFFREY A， et al. Angiotensin Ⅱ mediates the axonal trafficking of tyrosine hydroxylase and dopamine β-hydroxylase mRNAs and enhances norepinephrine synthesis in primary sympathetic neurons ［J］. Journal of Neurochemistry， 2019， 150（6）： 666-677.

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Related Author

Ailong SHA

Haiyan HAO

Related Institution

School of Biology and Food Engineering， Chongqing Research Center for the Development and Utilization of Northeast Chongqing Characteristic Biological Resources， Chongqing Three Gorges University

School of Environmental and Chemical Engineering， Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservoir Area， Chongqing Three Gorges University

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The mechanism of Suaeda rigida polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus

DOI：10.13471/j.cnki.acta.snus.2020.05.12.2020E016

摘要

Abstract

关键词

Keywords

references