YOU Yang,ZHANG Xiaoyun.Effects of low temperature on mesophyll cell ultrastructure of Osmanthus fragrans ‘Wandian Jin’[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2021,60(04):34-41.
YOU Yang,ZHANG Xiaoyun.Effects of low temperature on mesophyll cell ultrastructure of Osmanthus fragrans ‘Wandian Jin’[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2021,60(04):34-41. DOI: 10.13471/j.cnki.acta.snus.2020.03.06.2020E004.
Effects of low temperature on mesophyll cell ultrastructure of Osmanthus fragrans ‘Wandian Jin’
To understand the effects of low temperature stress on lysosomes or another organella in the mesophyll cell of
Osmanthus fragrants
‘Wandian Jin’, we chose three years old
O. fragrans
‘Wandian Jin’ as the test material. The intelligent artificial climate chamber was applied to simulate the natural cooling process for
O. fragrans
. After sampling and making slices, the mesophyll cell of
O
.
fragrans
was used for investigation with the transmission electron microscope. The results showed that the chloroplast and mitochondrion's structures did not display abnormal, however, the number of lysosomes decreased. Under the treatment of the low temperature at 5 ℃, some chloroplasts occurred a degree of swelling compared with that at the normal temperature; the number of osmiophilic granules and mitochondrions was increased; primary lysosome was found to coexist with secondary lysosome and their numbers grew. At low temperatures 0 ℃, chloroplast was more and more expand, some chloroplasts showed a spheroidicity shape; the amount of osmiophilic granule was significant; starch grain swelled and enlarged; the number of mitochondrion was increased; the structure of secondary lysosome was complicated and varying in size. At low temperatures -5 ℃, chloroplast swelled more strongerly than in the early, parts of the chloroplast were dissociated in the center of cells; the number of the mitochondrion, the primary lysosome and secondary lysosome was increased, and the shapes of lysosome varied. In addition, the mesophyll cells showed to ice intracellularly ice and freeze intercellularly.
关键词
桂花Osmanthus fragrans低温胁迫溶酶体超微结构
Keywords
Osmanthus fragranslow temperature stresslysosomeultrastructure
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