Protective effects of communic acid against the TBHP-induced oxidative stress injury in SH-SY5Y nerve cells

YAN Li; HE Xiang; MA Zekang; LI Peibo; SU Weiwei

doi:10.13471/j.cnki.acta.snus.2020.03.006

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Protective effects of communic acid against the TBHP-induced oxidative stress injury in SH-SY5Y nerve cells

更新时间：2023-12-11

- Protective effects of communic acid against the TBHP-induced oxidative stress injury in SH-SY5Y nerve cells
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 59, Issue 3, Pages: 51-58(2020)
- 作者机构：
  
  中山大学生命科学学院/广东省中药上市后质量与药效再评价工程技术研究中心,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2020.03.006
  CLC：
- Published：2020，
  
  Published Online：25 May 2020，
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YAN Li, HE Xiang, MA Zekang, et al. Protective effects of communic acid against the TBHP-induced oxidative stress injury in SH-SY5Y nerve cells. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 59(3):51-58(2020)
DOI：

YAN Li, HE Xiang, MA Zekang, et al. Protective effects of communic acid against the TBHP-induced oxidative stress injury in SH-SY5Y nerve cells. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 59(3):51-58(2020) DOI： 10.13471/j.cnki.acta.snus.2020.03.006.

摘要

采用TBHP处理SH-SY5Y细胞的方法，建立了细胞氧化应激损伤模型用以评价樱柏酸对细胞氧化应激损伤的保护作用及机制。同时借助MTS法、β-半乳糖苷酶（SA-β-gal）染色法、乳酸脱氢酶（LDH）含量检测法、Annexin V-FITC/PI 双染法、2′

，

7′-二氯荧光素二乙酸盐（DCFH-DA）法以及酶联免疫法（ELISA）法等对细胞的活性及衰老相关指标进行了检测。研究结果显示樱柏酸对TBHP诱导的SH-SY5Y氧化应激损伤细胞模型具有保护作用，并且能够降低细胞衰老比例、细胞凋亡水平，其作用机制可能通过减轻细胞，膜和DNA等氧化应激损伤、提高体内抗氧化酶SOD和GSH-Px酶活力减少ROS含量起效。

Abstract

The aim of this study was to investigate the neuroprotective effects of communic acid against cell oxidative stress injury induced by t-butylhydroperoxide (TBHP) in human neuroblastoma cells and its mechanisms. Meanwhile

MTS method

SA-glycosyl lactate dehydrogenase (LDH) staining method

Annexin v-fitc /PI double dyeing method

2′

7′- dichlorofluorescein diacetate (dcfh-da) method and Enzyme-linked immunosorbent assay (ELISA) was used to detect the activity and senescence of cells.The results showed that communic acid has a protective effect on TBHP=induced oxidative damage in SH-SY5Y cells

and can reduce the cell senescence ratio and the apoptosis level. The mechanism may be that TBHP reduced oxidative damage in cell membrane and DNA

and decreased the level of ROS content by improving enzyme activities such as antioxidant enzymes SOD.

关键词

樱柏酸SH-SY5Y细胞氧化应激叔丁基过氧化氢细胞损伤

Keywords

communic acidSH-SY5Y celloxidative stressT-butyhydroperoxidecell injury

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School of Pharmaceutical Sciences, Sun Yat-sen University

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