Vincetoxicum hainanense

is a national level Ⅱ rare and endangered plant in China. It has important ecological value. In this study

we selected three main factors

cutting slips

cutting medium and growth hormone that affect the seedling rate of V. hainanense

each factor included 3 levels

the optimum cutting propagation conditions were obtained by orthogonal test consisting of 3 factors and 3 levels. Results showed that: The seedling rate was highest by taking level 3 branches as cutting slips and bamboo grove soil with humus as cutting medium and 0.5 g/L NAA as growth hormone. With regular watering

adventitious roots emerged 7~10 d later and cuttings became seedling 12~15 d later

the seedling rate reached 87.0% after 1 month. We transplanted these seedlings in three natural distribution areas to do a reintroduction test. These areas are primeval forest of V. hainanense

secondary forest of Cinnamomum burmanni and secondary forest of Syzygium jambos. The results showed that reintroduction plants grew very well in primeval forest of V. hainanense

the average survival rate was 967%. After 6 month

the height of plant reached 252.0 cm

base diameter of plant reached 0.78 cm and branch number of the main stem was 3.7

after 9 month

reintroduction plants would blossom and set fruit

the flowering rate and fruit setting rate was 45.5% and 32.7%

it is a feasible method of reintroduction.45.5% and the seed setting rate 32.7%. Together

it can be concluded that it  is a feasible method of ecological restoration.